Serotonin N-acetyltransferase Antibody

Images

 
Western Blot: Serotonin N-acetyltransferase Antibody [NB100-135] - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.
Immunocytochemistry/ Immunofluorescence: Serotonin N-acetyltransferase Antibody [NB100-135] - AANAT in COS7 and 1E7 cells detected by ICC. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; ...read more

Product Details

Summary
Reactivity Hu, Pm, Bv(-), Ch(-), Rt(-), Ze(-)Species Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Serotonin N-acetyltransferase Antibody Summary

Immunogen
Human Serotonin N-acetyltransferase [UniProt# Q16613]
Specificity
This is specific for primate AANAT (3,4).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AANAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:200
Application Notes
This Serotonin N-acetyltransferase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot, this antibody recognizes a band at 23 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-135.

Reactivity Notes

Human and rhesus macaque. The antibody does not recognize rat, bovine, chicken or zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Eluted in 0.1N glacial acetic acid containing 0.1% BSA and neutralized using 10x PBS
Preservative
0.05% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Serotonin N-acetyltransferase Antibody

  • AA-NAT
  • aralkylamine N-acetyltransferaseEC 2.3.1.87
  • arylalkylamine N-acetyltransferase
  • Serotonin acetylase
  • SNATserotonin N-acetyltransferase

Background

Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function, and acts as"the hormone of the night." Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina (1,2). Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serotonin N-acetyltransferase Antibody (NB100-135)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Serotonin N-acetyltransferase Antibody (NB100-135). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know if this antibody can be used for bovine samples?
    • Our Serotonin N-acetyltransferase antibody NB100-135 cannot be used in bovine samples. We have evidence that it will not detect the bovine protein.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-135AF405
Alexa Fluor 488 Labeled NB100-135AF488
Alexa Fluor 647 Labeled NB100-135AF647
Alexa Fluor 700 Labeled NB100-135AF700
Biotin Labeled NB100-135B
DyLight 488 Labeled NB100-135G
DyLight 550 Labeled NB100-135R
DyLight 650 Labeled NB100-135C
HRP Labeled NB100-135H

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Bioinformatics

Gene Symbol AANAT
Entrez
Uniprot