Serotonin N-acetyltransferase Antibody

Western Blot: Serotonin N-acetyltransferase Antibody [NB100-135] - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.

Immunocytochemistry/Immunofluorescence: Serotonin N-acetyltransferase Antibody [NB100-135] - AANAT in COS7 and 1E7 cells detected by ICC. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.

Product Discontinued

Serotonin N-acetyltransferase Antibody Summary

• Immunogen: Human Serotonin N-acetyltransferase [UniProt# Q16613]
• Specificity: This is specific for primate AANAT (3,4).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AANAT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:500-1:1000
• Application Notes: This Serotonin N-acetyltransferase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot, this antibody recognizes a band at 23 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and rhesus macaque. The antibody does not recognize rat, bovine, chicken or zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Eluted in 0.1N glacial acetic acid containing 0.1% BSA and neutralized using 10x PBS
• Preservative: 0.05% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Serotonin N-acetyltransferase Antibody

• AA-NAT
• aralkylamine N-acetyltransferaseEC 2.3.1.87
• arylalkylamine N-acetyltransferase
• Serotonin acetylase
• SNATserotonin N-acetyltransferase

Background

Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function, and acts as"the hormone of the night." Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina (1,2). Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Serotonin N-acetyltransferase Antibody (NB100-135)(5)

Publications using NB100-135

• Klein, DC. Arylalkylamine N-acetyltransferase: the Timezyme. J. Biol Chem. 282(7):4233-7. 2007-02-16 [PMID: 17164235]
• Coon, SL et al. Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87). J Clin Endocrinol Metab. 87(10):4699-706. 2002-10-01 [PMID: 12364461]
• Klein, DC et al. 14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin. Biochem Soc Trans;30(4):365-73. 2002-08-01 [PMID: 12196096]
• Iuvone, PM et al. Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase. Invest Ophthalmol Vis Sci;43(2):564-72. 2002-02-01 [PMID: 11818405]
• Coon, SL et al. cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation. J Biol Chem. 276(26):24097-107. 2001-06-29 [PMID: 11313340]

FAQs for Serotonin N-acetyltransferase Antibody (NB100-135). (Showing 1 - 1 of 1 FAQs).

1. We would like to know if this antibody can be used for bovine samples?
   • Our Serotonin N-acetyltransferase antibody NB100-135 cannot be used in bovine samples. We have evidence that it will not detect the bovine protein.

Bioinformatics

• Gene Symbol: AANAT
• Entrez:
  • 15(Human)
  • 25120(Rat)
• Uniprot:
  • Q16613(Human)