Septin-9 Antibody

Images

 
Western Blot: Septin-9 Antibody [NBP1-28711] - Lane 1: marker. Lane 2: 10mg of HeLa whole cell lysate probed with NBP1-28711 antibody 1:1000 in PBST. Blot blocked with 5% milk in PBS with 0.1% tween 20 probed overnight ...read more
Immunocytochemistry/ Immunofluorescence: Septin-9 Antibody [NBP1-28711] - HeLa cells fixed with PFA (4%, 20 min, RT) with anti septin 9 antibody NBP1-28711 at 1:1000. Left panel: Control localisation to sub nuclear ...read more
Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP1-28711] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Septin 9 used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent ...read more
Immunoprecipitation: Septin-9 Antibody [NBP1-28711] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Septin 9 antibody NBP1-28711 used for IP at 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Septin-9 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 536 and 586 of human septin 9 using the numbering given in entry NP_001106963.1 (GeneID 10801).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEPT9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Septin-9 antibody validated for WB, ICC/IF from verified customer reviews.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-28711 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Septin-9 Antibody

  • AF17q25
  • FLJ75490
  • KIAA0991Ov/Br septin
  • MLL septin-like fusion protein MSF-A
  • MLL septin-like fusion protein
  • MSF1NAPB
  • MSFMLL septin-like fusion
  • Ovarian/Breast septin
  • PNUTL4
  • SeptD1
  • septin 9
  • Septin D1
  • septin-9
  • SINT1

Background

Septin 9 is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Septin-9 Antibody (NBP1-28711) (0)

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Reviews for Septin-9 Antibody (NBP1-28711) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-28711:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry Septin-9 NBP1-28711
Enlarge
5
reviewed by:
Stephen Terry
ICC Human 10/25/2018
View

Summary

ApplicationImmunocytochemistry
Sample Tested hela cell
SpeciesHuman
LotA1

Comments

CommentsCells fixed with 4% PFA for 20 minutes room temperature.

Fixed cells incubated with with 0.5% triton x100 in 1xPBS for 5 minutes room.

Washed twice in blocking buffer (1% BSA 1xPBS, 20mM glycine ).

Washed once with blocking buffer left for 1hour room temperature.

incubated with Anti septin 9 antibody in blocking buffer 1:1000 for 20 hours at 4 degrees.

Washed 3 x 5 minutes in blocking buffer

Incubated with Anti rabbit-Alexa 594 conjugated antibody (Jackson immunoscience) for 2 hours room temperature.

Washed 3 x 5 minutes in blocking buffer

Washed 1 x 5 minutes in PBS

Imaged with 60X oil objective on Nikon Eclipse Ti Inverted Fast confocal - Equipped with a Yokogawa CSU-X1 spinning disk unit and okogawa CSU-1 disk head and Andor Neo sCMOS camera.
Western Blot Septin-9 NBP1-28711
Enlarge
4
reviewed by:
Stephen Terry
WB Human 10/22/2018
View

Summary

ApplicationWestern Blot
Sample Tested Hela whole cell lysate
SpeciesHuman
LotA1

Comments

Comments10mg of HeLa whole cell lysate was loaded run on a 4-12% bi-Tris acrylamide gell run in 1X mops buffer.

Blot blocked with 5%milk in PBS with 0.1% tween 20 probed overnight at 4 degrees C with NBP1-28711 1:1000 washed 3X PBST incubated with anti rabbit HRP secondary washed 3X PBST, developed with ECL prime kit GE healthcare.

Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

FAQs for Septin-9 Antibody (NBP1-28711) (0)

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Secondary Antibodies

 

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Stephen Terry
10/25/2018
Application: ICC
Species: Human

Stephen Terry
10/22/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol SEPT9
Entrez
Uniprot