Septin-9 Antibody

Western Blot: Septin-9 Antibody [NBP1-28711] - Lane 1: marker. Lane 2: 10mg of HeLa whole cell lysate probed with NBP1-28711 antibody 1:1000 in PBST. Blot blocked with 5% milk in PBS with 0.1% tween 20 probed overnight at 4C with NBP1-28711 at 1:1000 washed 3X PBST incubated with anti rabbit HRP secondary antiboy, washed 3X PBST, developed with ECL prime kit GE healthcare. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP1-28711] - HeLa cells fixed with PFA (4%, 20 min, RT) with anti septin 9 antibody NBP1-28711 at 1:1000. Left panel: Control localisation to sub nuclear filaments. Right panel: Cells treated with cytochalasin D to depolymerize F-actin. Note localization to ring like structures. Image from verified customer review.

Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP1-28711] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Septin 9 used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

Immunoprecipitation: Septin-9 Antibody [NBP1-28711] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Septin 9 antibody NBP1-28711 used for IP at 3 ug/mg lysate. Septin 9 was also immunoprecipitated by rabbit anti-Septin 9 antibody NBP1-28764, which recognizes an upstream epitope. For blotting immunoprecipitated Septin 9, NBP1-28764 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Septin-9 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 536 and 586 of human septin 9 using the numbering given in entry NP_001106963.1 (GeneID 10801).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEPT9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot
• Application Notes: Septin-9 antibody validated for WB, ICC/IF from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Septin-9 Antibody

• AF17q25
• FLJ75490
• KIAA0991Ov/Br septin
• MLL septin-like fusion protein MSF-A
• MLL septin-like fusion protein
• MSF1NAPB
• MSFMLL septin-like fusion
• Ovarian/Breast septin
• PNUTL4
• SeptD1
• septin 9
• Septin D1
• septin-9
• SINT1

Background

Septin 9 is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Septin-9 Antibody (NBP1-28711) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 10/25/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: hela cell
• Species: Human
• Lot: A1

Comments

• Comments: Cells fixed with 4% PFA for 20 minutes room temperature.
  
  Fixed cells incubated with with 0.5% triton x100 in 1xPBS for 5 minutes room.
  
  Washed twice in blocking buffer (1% BSA 1xPBS, 20mM glycine ).
  
  Washed once with blocking buffer left for 1hour room temperature.
  
  incubated with Anti septin 9 antibody in blocking buffer 1:1000 for 20 hours at 4 degrees.
  
  Washed 3 x 5 minutes in blocking buffer
  
  Incubated with Anti rabbit-Alexa 594 conjugated antibody (Jackson immunoscience) for 2 hours room temperature.
  
  Washed 3 x 5 minutes in blocking buffer
  
  Washed 1 x 5 minutes in PBS
  
  Imaged with 60X oil objective on Nikon Eclipse Ti Inverted Fast confocal - Equipped with a Yokogawa CSU-X1 spinning disk unit and okogawa CSU-1 disk head and Andor Neo sCMOS camera.

reviewed by: Verified Customer WB Human 10/22/2018

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: A1

Comments

• Comments: 10mg of HeLa whole cell lysate was loaded run on a 4-12% bi-Tris acrylamide gell run in 1X mops buffer .
  
  Blot blocked with 5%milk in PBS with 0.1% tween 20 probed overnight at 4 degrees C with NBP1-28711 1:1000 washed 3X PBST incubated with anti rabbit HRP secondary washed 3X PBST , developed with ECL prime kit GE healthcare.

Bioinformatics

• Gene Symbol: SEPT9
• Entrez:
  • 10801(Human)
• Uniprot:
  • NP_001106963.1(Human)