SENP7 Antibody - BSA Free

Western Blot: SENP7 Antibody [NBP1-50027] - Detection of Human SENP7 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SENP7 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SENP7 was also immunoprecipitated by rabbit anti-SENP7 antibody BL10697, which recognizes an upstream epitope. For blotting immunoprecipitated SENP7, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

• Reactivity: Hu, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SENP7 Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 650 and 700 of human SUMO1/Sentrin Specific Peptidase 7 using the numbering given in entry EAW79800.1 (GeneID 57337).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SENP7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SENP7 Antibody - BSA Free

• EC 3.4.22.-
• KIAA1707SUMO1/sentrin specific protease 7
• MGC157730
• Sentrin/SUMO-specific protease SENP7
• sentrin-specific protease 7
• SSP2
• SUMO1/sentrin specific peptidase 7
• SUMO-1-specific protease 2
• SUSP2

Background

SAE1, or SUMO-1 specific protease 2 is a ubiquitin-like (Ubl) protein that has been shown to bind to several protiens including Ran GTPase-activating protein 1 (RanGAP1), IkappaBalpha, and PML. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SENP7 Antibody (NBP1-50027)(1)

Publication using NBP1-50027

• Ji P, Liang S, Li P et al. Speckle-type POZ protein suppresses hepatocellular carcinoma cell migration and invasion via ubiquitin-dependent proteolysis of SUMO1/sentrin specific peptidase 7 Biochem. Biophys. Res. Commun. 2018-05-24 [PMID: 29777712] (WB, Human)

Bioinformatics

• Gene Symbol: SENP7
• Entrez:
  • 57337(Human)
• Uniprot:
  • Q9BQF6(Human)