Semaphorin 4C Antibody (660016)

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human Semaphorin 4C Monoclonal Antibody (Catalog # MAB6125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Semaphorin 4C at approximately 100 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 660016
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Semaphorin 4C Antibody (660016) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C
  Ala21-Gly663 (predicted)
  Accession # Q9C0C4
• Specificity: Detects human Semaphorin 4C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, 7A, recombinant mouse Semaphorin 3B, 3C, 3E, 3F, 4A, 4B, 4C, 4D, 4F, 4G, 6A, 6B, 6C, 6D, or 7A is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEMA4C
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4C Antibody (660016)

• FLJ20369
• KIAA1739
• MGC126382
• MGC126383
• M-Sema F
• M-SEMA-F
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C
• SEMA4C
• SEMACL1
• Semaf
• SEMAI
• Semaphorin 4C
• semaphorin-4C

Background

Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins.  It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms.  Mature human Semaphorin 4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length.  It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499-551), and an Ig-like C2-type domain (aa 556-644).  The cytoplasmic region interacts with PZD-domain containing proteins.  There are three potential splice variants.  One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36.  Over aa 21-663, human Semaphorin 4C shares 85% aa identity with mouse Semaphorin 4C.                       

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 4C Antibody (MAB6125)(1)

Publication using MAB6125

• Ya Wang, Long Qiao, Jie Yang, Xiong Li, Yaqi Duan, Jiahao Liu et al. Serum semaphorin 4C as a diagnostic biomarker in breast cancer: A multicenter retrospective study Cancer Communications 2021-12-01 [PMID: 34738326]

Bioinformatics

• Gene Symbol: SEMA4C
• Uniprot:
  • Q9C0C4(Human)