Semaphorin 4C Antibody (660016) [Alexa Fluor® 405]

• Applications: WB
• Clone: 660016
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Semaphorin 4C Antibody (660016) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C
  Ala21-Gly663 (predicted)
  Accession # Q9C0C4
• Specificity: Detects human Semaphorin 4C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, 7A, recombinant mouse Semaphorin 3B, 3C, 3E, 3F, 4A, 4B, 4C, 4D, 4F, 4G,
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins.  It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms.  Mature human Semaphorin 4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length.  It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499-551), and an Ig-like C2-type domain (aa 556-644).  The cytoplasmic region interacts with PZD-domain containing proteins.  There are three potential splice variants.  One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36.  Over aa 21-663, human Semaphorin 4C shares 85% aa identity with mouse Semaphorin 4C.                       

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.