Semaphorin 3C Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Semaphorin 3C Antibody [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3C
  Gln24-Ser751 (Arg48Ala, Arg52Ala)
  Accession # Q62181
• Specificity: Detects mouse Semaphorin 3C in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Semaphorin 3A, rmSemaphorin 3B, rmSemaphorin 3E, and rmSemaphorin 3F is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 3C Antibody [Alexa Fluor® 750]

• (semaphorin) 3C
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema E
• SEMA3C
• SEMAE
• Semaphorin 3C
• Semaphorin E
• semaphorin-3C
• semaphorin-E
• SEME

Background

Semaphorin 3C (Sema 3C; previously semaE) is one of six Class 3 secreted semaphorins which share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 amino acid (aa) mouse Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1-3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Mouse Sema 3C shares at least 95% aa identity with human, rat, cow and dog Sema 3C, and 89% and 75% aa sequence identity with chick and zebrafish Sema 3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (1, 2). Sema 3C signaling is transduced by Plexin-D1 indirectly via neuropilin-1 or neuropilin-2 receptors (5). Sema 3C is expressed in all somitic motor neurons, in lung buds and in cardiac neural crest cells during development (1, 5-8). Sema 3C activates integrins in certain cells so, in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema 3A repulsion in adjacent cell layers (1, 6, 7). Sema 3C also provides an attractive force opposing Sema 6A and Sema 6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema 3C or Plexin-D1 genes or Sema 3C-neuropilin interactions are disrupted (5, 11, 12).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.