Semaphorin 3B Antibody (904201) [Unconjugated]

HAF005). A specific band was detected for Semaphorin 3B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF005). A specific band was detected for Semaphorin 3B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Semaphorin 3B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." > NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." title="Semaphorin 3B was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B (upper panel) or irrelevant transfectant (lower panel) using Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." />

Semaphorin 3B was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B (upper panel) or irrelevant transfectant (lower panel) using Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clone: 904201
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Semaphorin 3B Antibody (904201) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3B
  Pro26-Pro722
  Accession # Q62177
• Specificity: Detects mouse Semaphorin 3B in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SEMA3B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 3B Antibody (904201) [Unconjugated]

• (semaphorin) 3B
• LUCA-1
• Sema A(V)
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema V
• SemA
• SEMA3B
• SEMA5
• SEMA5FLJ34863
• SEMAA
• Semaphorin 3B
• semaphorin A
• semaphorin V
• semaphorin-3B
• semaphorin-V
• SEMAV

Background

Sema3b (Semaphorin 3B) is a 748 aa secreted glycoprotein.  Mouse Sema3b shares 88% and 96% aa identity with human and rat Sema3b, respectively.  The semaphorin family has been demonstrated to play a role in axon guidance.  Additionally, Sema3b has been implicated in bone mass regulation and tumor suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 3B Antibody (MAB5440)(1)

Publication using MAB5440

• M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1 Nat Commun, 2019-07-26;10(1):3345. 2019-07-26 [PMID: 31350404] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: SEMA3B
• Uniprot:
  • Q62177(Mouse)