SEC23A Antibody

Western Blot: SEC23A Antibody [NBP1-47201] - Western blot analysis of SEC23A in NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-47201 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu, Rt, Bv, Ca, Eq
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SEC23A Antibody Summary

• Description: Novus Biologicals Goat SEC23A Antibody (NBP1-47201) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-SEC23A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ANRAATTGHVID corresponding to internal region according to NP_006355.2.
• Epitope: internal region
• Predicted Species: Human (100%), Rat (100%), Canine (100%), Equine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEC23A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 100 kDa band observed in lysates of NIH3T3 (calculated MW of 86.2 kDa band according to human NP_006355.2 and to mouse NP_033173.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SEC23A Antibody

• CLSD
• MGC26267
• protein transport protein Sec23A
• Sec23 (S. cerevisiae) homolog A
• Sec23 homolog A (S. cerevisiae)
• SEC23-related protein A

Background

SEC23A is encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family. It is part of a protein complex and found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles. This protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The encoded protein is suggested to play a role in the ER-Golgi protein trafficking. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SEC23A Antibody (NBP1-47201)(2)

Publications using NBP1-47201

• Stomberski CT, Venetos NM, Zhou HL et al. A multienzyme S-nitrosylation cascade regulates cholesterol homeostasis Cell reports 2022-10-25 [PMID: 36288700] (ICC/IF, Human)
• Bi X, Mancias JD, Goldberg J. Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexedwith the active fragment of Sec31. Dev Cell 2007-11-01 [PMID: 17981133]

Bioinformatics

• Gene Symbol: SEC23A
• Entrez:
  • 10484(Human)
  • 20334(Mouse)
• Uniprot:
  • Q15436(Human)
  • Q01405(Mouse)