SDS3 Antibody

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Biological Strategies: Western Blot: SDS3 Antibody [NB100-257] - Detection of Human and Mouse SDS3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN ...read more
Independent Antibodies: Immunoprecipitation: SDS3 Antibody [NB100-257] - Detection of human SDS3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, PmSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SDS3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 175 and 225 of human Suppressor of Defective Silencing 3 using the numbering given in entry NP_071936.2 (GeneID 64426).
Predicted Species
Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SUDS3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000-1:5000
Application Notes
By Western blot, this antibody recognizes bands around 45 kDa representing SDS3.
Control
HeLa Whole Cell Lysate
SDS3 Overexpression Lysate
Publications
Read Publications using NB100-257.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SDS3 Antibody

  • 45 kDa Sin3-associated polypeptide
  • FLJ00052
  • SAP45suppressor of defective silencing 3 homolog (SDS3, S. cerevisiae)
  • SDS3MGC104711
  • sin3 histone deacetylase corepressor complex component SDS3
  • sin3A-associated protein, 45kDa
  • suppressor of defective silencing 3 homolog (S. cerevisiae)
  • Suppressor of defective silencing 3 protein homolog

Background

SDS3 is a subunit of the histone deacetylase-dependent SIN3A corepressor complex. The SIN3A corepressor complex functions to repress transcription through its interaction with histone deacetylases HDAC1 and HDAC2. SDS3 has been demonstrated to play a role in pericentric heterochromatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SDS3 Antibody (NB100-257)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SUDS3
Entrez
Uniprot