SDHA Antibody

Images

 
Western Blot: SDHA Antibody [NBP2-32594] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: SDHA Antibody [NBP2-32594] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
Immunohistochemistry: SDHA Antibody [NBP2-32594] - Staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SDHA Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ERASAKVSDSISAQYPVVDHEFDAVV
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SDHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:250
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SDHA Protein (NBP2-32594PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SDHA Antibody

  • CMD1GG
  • EC 1.3.5.1
  • Fp
  • SDH1
  • SDH2
  • SDHFmitochondrial
  • succinate dehydrogenase complex flavoprotein subunit
  • succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SDHA Antibody (NBP2-32594) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SDHA Products

Bioinformatics Tool for SDHA Antibody (NBP2-32594)

Discover related pathways, diseases and genes to SDHA Antibody (NBP2-32594). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SDHA Antibody (NBP2-32594)

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Pathways for SDHA Antibody (NBP2-32594)

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PTMs for SDHA Antibody (NBP2-32594)

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Research Areas for SDHA Antibody (NBP2-32594)

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Blogs on SDHA.

SDHA - oxidative enzyme in the citric acid cycle
Succinate dehydrogenase is an important tetrameric protein involved in the citric acid cycle. It is localized to the inner mitochondrial membrane of cells. Succinate dehydrogenase makes up Complex II of the electron transport chain (ETC) and is re...  Read full blog post.

SDHA - An essential Krebs cycle enzyme with role in cancer and metabolism
Succinate dehydrogenase (SDH) is a highly conserved protein complex located on the inner mitochondrial membrane where it functions during the Krebs cycle by oxidizing succinate to fumarate (1). This reaction is also important for feeding electrons...  Read full blog post.

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Bioinformatics

Gene Symbol SDHA