SDHA - An essential Krebs cycle enzyme with role in cancer and metabolism

Thu, 10/22/2015 - 14:35

Succinate dehydrogenase (SDH) is a highly conserved protein complex located on the inner mitochondrial membrane where it functions during the Krebs cycle by oxidizing succinate to fumarate (1). This reaction is also important for feeding electrons into the electron transport chain. SDH complex contains four subunits: SDH-A, -B, -C, and -D. Mutation of SDH-A often leads to mitochondrial encephalopathy while mutations to subunits B, C, and D lead to tumors of the head and neck (1).

Belinsky et al. performed immunohistochemistry using SDHA antibody to identify SDH complex deficiency in patient samples and found that bi-allelic inactivation of SDHA is common in gastrointestinal stromal tumors (2). Following staining with the SDHA antibody, samples were sequenced and verified to contain various inactivating mutations. This SDHA antibody based method of detecting mutations has proven to be a quick and efficient method for characterizing tumor samples without the cost or time associated with sequencing (3). In addition to its use as a biomarker or diagnostic tool, SDHA antibodies have been used to characterize the protein-protein interactions of succinate dehydrogenase (4). Finley et al. performed immunoprecipitation experiments to show SIRT3, a deacetylase with functions in metabolism, interacts directly with SDHA (4). Additionally, SDHA is a substrate of SIRT3. Together these studies demonstrate multiple uses for SDHA antibodies in clinical and basic research applications.

Novus Biologicals offers SDHA reagents for your research needs including:


  1. 12082502
  2. 23730622
  3. 21752896
  4. 21858060

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