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Mouse SDF2L1 ELISA Kit (Colorimetric)

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Mouse SDF2L1 ELISA Kit (Colorimetric) - Standard Curve Reference
Mouse SDF2L1 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

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Mouse SDF2L1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse SDF2L1 in samples. No significant cross-reactivity or interference between Mouse SDF2L1 and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.3%
Intra-Assay
CV% < 5.19%
Spike Recovery
85-102%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SDF2L1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Mouse SDF2L1 ELISA Kit (Colorimetric)

  • dihydropyrimidinase-like 2
  • PWP1-interacting protein 8
  • SDF2-like 1
  • stromal cell-derived factor 2-like 1
  • stromal cell-derived factor 2-like protein 1
  • UNQ1941/PRO4424

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Western Blot: CRMP2 Antibody [NBP1-85448] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-85448] - Staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.
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Glutamine
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Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
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Immunohistochemistry-Paraffin: SDF2 Antibody (3G7-1D6) [H00006388-M01] - Analysis of monoclonal antibody to SDF2 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.
Immunohistochemistry-Paraffin: SDF2 Antibody (3G7-1D6) [H00006388-M01] - Analysis of monoclonal antibody to SDF2 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.
Western Blot: SDF2 Antibody (3G7-1D6) [H00006388-M01] - SDF2 monoclonal antibody (M01), clone 3G7-1D6 Analysis of SDF2 expression in WI-38.
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Applications: ELISA, IHC, IHC-P, S-ELISA, WB
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Western Blot: DRP2 Antibody [NBP2-16229] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:500.
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Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.Lane 1: ENO1 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on HeLa cell. Antibody concentration 10 ug/ml.
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Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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Species: Ca, Ch, ChHa, Fe, Hu, Mu, Ma-Op, Po, Pm, Rt
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Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-47284] - Staining of human small intestine shows high expression.
Immunocytochemistry/Immunofluorescence: SDF4 Antibody [NBP2-47284] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.
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Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HMGB1 [NB100-2322] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G Sepharose beads. Tubes were rocked O/N at 4 degrees C followed by several washes to remove unbound antibodies. U2OS were collected in HEPES buffer (20 mM HEPES, 100 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, pH 7.4) supplemented with protease inhibitor. Lysates are rocked 30 min at 4 degrees C and spun at 110,000xg for 15 min at 4 degrees C. One ml aliquots at 1 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4 degrees C. Following centrifugation, the unbound fractions were collected, and beads were subsequently washed three times with 1 ml of HEPES lysis buffer and processed for SDS-PAGE and immunoblot. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).
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Applications: ELISA, Flow, IHC, IHC-Fr, IHC-P, IP, KO, Simple Western, WB
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Immunohistochemistry: alpha-Internexin Antibody (2E3) [NB300-140] - Section of rat facial nucleus 7 days following axotomy. These neurons are capable of regenerating their axons and also, concomitant with regeneration, strongly upregulate alpha-Internexin in their perikarya. Other central neurons which are not able to regenerate their axons do not upregulate this protein after axotomy and untreated facial neurons normally show only very low levels of alpha-Internexin. Both findings suggest that alpha-Internexin has a role in axonal regeneration.
Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody (2E3) [NB300-140] - Hippocampal neurons in tissue culture with neurofilament alpha internexin (red), using NB300-140 and GFAP (green), using NB300-141.
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BSA Free
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Western Blot: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in Jurkat whole cell extracts
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Immunohistochemistry: MYL6 Antibody [NBP1-54376] - Human Skeletal Muscle.
Immunohistochemistry: MYL6 Antibody [NBP1-54376] - Staining of human Prostate.
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Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli and cells in tubules.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human stomach shows moderate membranous positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P

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  Mouse SDF2L1 ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol SDF2L1