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Mouse SDF2L1 ELISA Kit (Colorimetric)

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Mouse SDF2L1 ELISA Kit (Colorimetric) - Standard Curve Reference
Mouse SDF2L1 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

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Mouse SDF2L1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse SDF2L1 in samples. No significant cross-reactivity or interference between Mouse SDF2L1 and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.3%
Intra-Assay
5.19%
Spike Recovery
85-102%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SDF2L1

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Certificate of Analysis
  2. Biotinylated Detection Ab Diluent
  3. HRP Conjugate Diluent
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Biotinylated Detection Ab (100×)
  6. Concentrated Wash Buffer (25×)
  7. Stop Solution
  8. Reference Standard & Sample Diluent
  9. Substrate Reagent
  10. Plate Sealer
  11. Micro ELISA Plate(Dismountable)
  12. Reference Standard
  13. Product Description

Alternate Names for Mouse SDF2L1 ELISA Kit (Colorimetric)

  • dihydropyrimidinase-like 2
  • PWP1-interacting protein 8
  • SDF2-like 1
  • stromal cell-derived factor 2-like 1
  • stromal cell-derived factor 2-like protein 1
  • UNQ1941/PRO4424

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Western Blot: CRMP2 Antibody [NBP1-85448] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: CRMP2 Antibody [NBP1-85448] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CRMP2 Antibody [NBP1-85448] - Staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Glutamine
Synthetase Antibody
NB110-41404
Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
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(4 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC
SDF2 Antibody
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Western Blot: SDF2 Antibody [NBP1-58376] - This Anti-SDF2 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1.25ug/ml.
Immunohistochemistry: SDF2 Antibody [NBP1-58376] - Analysis of Human kidney.
Species: Hu, Mu, Rt, Bv, Ca, Eq, Gt, GP, Rb, Ze
Applications: WB, IHC, IHC-P
DRP2 Antibody
NBP1-86525
Immunocytochemistry/Immunofluorescence: DRP2 Antibody [NBP1-86525] - Staining of human cell line AF22 shows localization to nuclear speckles & plasma membrane.
Species: Hu
Applications: ICC/IF
Enolase 1 Antibody
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Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8. Analysis of ENO1 expression in human colon.
Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.
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Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
14-3-3 Antibody
NBP2-27202
Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.
(1 Review)
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Op, Pm
Applications: WB, Simple Western
SDF4 Antibody
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Immunocytochemistry/Immunofluorescence: SDF4 Antibody [NBP2-47284] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-47284] - Staining in human small intestine and pancreas tissues using anti-SDF4 antibody. Corresponding SDF4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-47284] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
DNAJB11 Antibody
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Western Blot: DNAJB11 Antibody [NBP1-84899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: DNAJB11 Antibody [NBP1-84899] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84899] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Ferritin Heavy
Chain Antibody
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Western Blot: Ferritin Antibody [NBP1-31944] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.
Immunocytochemistry/Immunofluorescence: Ferritin Antibody [NBP1-31944] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Ferritin Antibody [NBP1-31944] - Paraffin-embedded mouse liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
HMGB1/HMG-1
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Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Total protein from SHSY-5Y, MCF7, Neuro2A and HeLa was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HMGB1 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody [NB100-2322] - HMGB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.05 ug/mL was used. Image objective 40x.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NB100-2322] - Rabbit blood vessel. Image from verified customer review.
(5 Reviews)
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Species: Hu, Mu, Rt, Bv, Ca, Rb, Sh
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beta-Actin
Antibody (AC-15)
NB600-501
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.
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POMT1 Antibody
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Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Rb
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CNPase Antibody
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Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.
Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Species: Hu, Mu
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alpha-Internexin
Antibody (2E3)
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Western Blot: alpha-Internexin Antibody (2E3) [NB300-140] - Analysis of different tissue lysates using alpha-Internexin, dilution 1:10000 (Red): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord lysate. The antibody reveals the alpha-Internexin protein with an apparent molecular weight of 64-66kDa, with some variability across species.
Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody (2E3) [NB300-140] - Hippocampal neurons in tissue culture with neurofilament alpha internexin (red), using NB300-140 and GFAP (green), using NB300-141.
Immunohistochemistry: alpha-Internexin Antibody (2E3) [NB300-140] - Section of rat facial nucleus 7 days following axotomy. These neurons are capable of regenerating their axons and also, concomitant with regeneration, strongly upregulate alpha-Internexin in their perikarya. Other central neurons which are not able to regenerate their axons do not upregulate this protein after axotomy and untreated facial neurons normally show only very low levels of alpha-Internexin. Both findings suggest that alpha-Internexin has a role in axonal regeneration.
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Species: Hu, Mu, Rt, Po, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
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Immunocytochemistry/Immunofluorescence: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in HeLa cells using NBP1-49532. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: PGAM1 Antibody [NBP1-49532] - Analysis of PGAM1 in mouse tongue.
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
MYL6 Antibody
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Western Blot: MYL6 Antibody [NBP1-54376] - Human 721_B, Antibody Dilution: 1.0 ug/ml MYL6 is supported by BioGPS gene expression data to be expressed in 721_B.
Immunohistochemistry: MYL6 Antibody [NBP1-54376] - Staining of human Prostate.
Western Blot: MYL6 Antibody [NBP1-54376] - HepG2 tissue lysate at a concentration of 1ug/ml.
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Sh, Ze
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Immunocytochemistry/Immunofluorescence: Utrophin Antibody [NBP1-84074] - Staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human liver shows no membranous positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.
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Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.
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  Mouse SDF2L1 ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol SDF2L1