Recombinant Human SCGF/CLEC11a Protein

Product Discontinued

Recombinant Human SCGF/CLEC11a Protein Summary

• Description:
  Gly19-Phe323, with an N-terminal Met and a C-terminal 6-His tag
  Accession # Q9Y240
• Details of Functionality: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF kit Ligand. The ED_50 for this effect is 0.12-0.6 ug/mL in the presence of cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
• Source: NS0
• Protein/Peptide Type: Biologically Active Protein
• Gene: CLEC11A
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 ug/mL in PBS containing at least 0.1% human or bovine serum albumin.

Alternate Names for Recombinant Human SCGF/CLEC11a Protein

• CLEC11a
• CLECSF3
• LSLCL
• P47
• SCGF

Background

Stem Cell Growth Factor (SCGF), also known as LSLCL, P47, and CLEC11A, is a secreted glycoprotein that acts on primitive hematopoietic stem/progenitor cells to support their proliferation and differentiation. Mature human SCGF contains an N-terminal Glu-rich region with an RGD motif, a leucine zipper region, and a C-terminal lectin-like domain (1, 2). The RGD motif is absent in mouse and rat SCGF (1). Full length human SCGF (the alpha isoform) shares approximately 83% amino acid (aa) sequence identity with mouse and rat SCGF. A shorter SCGF beta isoform lacks 78 aa of the lectin domain (1-3). This recombinant protein corresponds to the SCGF alpha isoform. SCGF is produced by various hematopoietic cell lines and by CD34^+ and CD34-CD33^+ bone marrow cells as a 47 kDa sulfated and O-glycosylated sialoprotein (2-6). It is also expressed by infiltrating macrophages in some gastrointestinal tract tumors (7). Mouse SCGF mRNA has been detected in proliferating chondrocytes, the primary ossification center, perichondrium, and periosteum (6). SCGF should not be confused with the similarly named SCF (stem cell factor)/c-Kit Ligand which exerts distinct activities during hematopoietic differentiation. SCGF exhibits erythroid burst (BFU-E) promoting activity in the absence of SCF/c-kit Ligand but suppresses the formation of erythroid bursts induced by SCF (4, 6). SCGF cooperates with IL-3, Flt-3 Ligand, GM-CSF, or G-CSF to enhance the growth of granulocyte-macrophage colonies (CFU-GM) (4, 6). It also promotes (with Flt-3 Ligand and VEGF) the maintenance and expansion of CD34^+CD133^+ progenitor cells as well as their differentiation into the endothelial lineage (8, 9). Serum levels of SCGF increase during stem cell transplantation and correlate with the recovery of hematopoiesis (5). Circulating SCGF levels are decreased in severe malarial anemia, and an SCGF promoter polymorphism (-539C/T) is associated with resistance to malarial anemia (10, 11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC11A