SCFD1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SCFD1 Antibody [NBP1-88418] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Analysis in human cell line TD47D.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SCFD1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SCFD1 Antibody - BSA Free (NBP1-88418) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AALAASAVTQVAKVFDQYLNFITLEDDMFVLCNQNKELVSYRAINRPDITDTEMETVMDTIVDSLFCFFVTLGAVPIIRCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDTLGAGQFSFQRPL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCFD1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SCFD1 Protein (NBP1-88418PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SCFD1 Antibody - BSA Free

• C14orf163
• KIAA0917chromosome 14 open reading frame 163
• sec1 family domain containing 1
• sec1 family domain-containing protein 1
• SLY1 homolog
• SLY1
• sly1p
• STXBP1L2RA410
• Syntaxin-binding protein 1-like 2
• vesicle transport-related protein

Background

SCFD1 (sec1 family domain containing 1) belongs to the STXBP-unc/SEC1 family of proteins. SEC1 proteins are involved in synaptic transmission and general secretion. SCFD1 is proposed to play a role in vesicular transport between the endoplasmic reticulum and the Golgi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCFD1