VPS45 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - Rat spinal cord using Rabbit antibody to internal part of Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a): IgG at a concentration of 10 ug/ml.

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-98931] - IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VPS45 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit VPS45 Antibody - Azide and BSA Free (NB100-98931) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from rat VPS45 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous with the corresponding sequence in mouse.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS45
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-50 ug/ml
  • Immunohistochemistry-Frozen 10-50 ug/ml
  • Immunohistochemistry-Paraffin 10-50 ug/ml
  • Western Blot 10-50 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterilized water
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for VPS45 Antibody - Azide and BSA Free

• H1
• H1VPS45
• hlVps45
• h-vps45
• leucocyte vacuolar protein sorting 45
• vacuolar protein sorting 45 homolog (S. cerevisiae)
• vacuolar protein sorting 45A (yeast homolog)
• vacuolar protein sorting 45A (yeast)
• vacuolar protein sorting 45A
• vacuolar protein sorting-associated protein 45
• VPS45A
• VPS45AVPS54A
• VPS45B
• VSP45
• VSP45A

Background

FUNCTION: May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.; SUBUNIT: Interacts with STX6 and ZFYVE20. SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network. TISSUE SPECIFICITY: Ubiquitous; expression was highest in testis and in brain. Detected in every part of the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPS45
• Entrez:
  • 22365(Mouse)
  • 64516(Rat)
• Uniprot:
  • O08700(Rat)