Recombinant Mouse SCF/c-kit Ligand Protein

Product Discontinued

Recombinant Mouse SCF/c-kit Ligand Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 26-189 of Mouse SCF/c-kit Ligand
  
  

  Source: E.coli

  Amino Acid Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

• Details of Functionality: Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is less or equal to 20 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: KITLG
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse SCF/c-kit Ligand Protein

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Mouse SCF (Stem cell factor, steel factor, Kit ligand, C-kit-ligand) is a hematopoietic growth factor that stimulates the proliferation of bone marrow stem cells. SCF induces the proliferation of mast cells and primitive lymphoid and myeloid hematopoietic bone marrow progenitor cells in culture. SCF acts synergistically with both GM-CSF and G-CSF, IL-7 and EPO. Mouse SCF is active on human cells as well as murine cells and can be used for long term bone marrow culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: KITLG