Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

ELISA: SCF/c-kit Ligand ELISA Kit [HRP] [KA1022] - Standard curve

Product Discontinued

Human SCF/c-kit Ligand ELISA Kit (Colorimetric) Summary

• Description: KITLG (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human KITLG in plasma, serum, and cell culture supernatants.
• Standard Curve Range: 0.156 to 40 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.20%
• Intra-Assay: CV% < 4.50%
• Spike Recovery: 96%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: KITLG

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human SCF/c-kit Ligand ELISA Kit (Colorimetric)

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Mouse SCF (Stem cell factor, steel factor, Kit ligand, C-kit-ligand) is a hematopoietic growth factor that stimulates the proliferation of bone marrow stem cells. SCF induces the proliferation of mast cells and primitive lymphoid and myeloid hematopoietic bone marrow progenitor cells in culture. SCF acts synergistically with both GM-CSF and G-CSF, IL-7 and EPO. Mouse SCF is active on human cells as well as murine cells and can be used for long term bone marrow culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for SCF/c-kit Ligand ELISA Kit (KA1022)(4)

Publications using KA1022

• Perumal D, Pillai S, Nguyen J et al. Nicotinic acetylcholine receptors induce c-Kit ligand/Stem Cell Factor and promote stemness in an ARRB1/ beta-arrestin-1 dependent manner in NSCLC. Oncotarget. 2014-11-15 [PMID: 25401222] (ELISA, Human)
• Levina V, Marrangoni A, Wang T et al. Elimination of human lung cancer stem cells through targeting of the stem cell factor-c-kit autocrine signaling loop. Cancer Res. 2010-01-01 [PMID: 20028869]
• Piao CS, Gonzalez-Toledo ME, Xue YQ et al. The role of stem cell factor and granulocyte-colony stimulating factor in brain repair during chronic stroke. J Cereb Blood Flow Metab. 2009-04-01 [PMID: 19209180]
• Al-Muhsen SZ, Shablovsky G, Olivenstein R et al. The expression of stem cell factor and c-kit receptor in human asthmatic airways. Clin Exp Allergy. 2004-06-01 [PMID: 15196279]

FAQs for SCF/c-kit Ligand ELISA Kit (KA1022). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: KITLG