SCF/c-kit Ligand Antibody [Alexa Fluor® 350]

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

SCF/c-kit Ligand Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant rat SCF/c-kit Ligand
  Gln26-Ala189
  Accession # P21581
• Specificity: Detect mouse and rat SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human SCF/c-kit Ligand is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KITLG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody [Alexa Fluor® 350]

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

SCF (Stem Cell Factor; also kit ligand and MGF) is a 40-45 kDa member of the SCF family of molecules. It is expressed by multiple cell types, including Sertoli cells, neurons, select fibroblasts, mast cells, melanocytes and dendritic cells. SCF binds to SCFR/c-kit (for kitten; the animal used to isolate it), and occurs in both a soluble and membrane-bound form. The soluble form of SCF produces a transient activation of SCFR, while the membrane-bound form generates a long-lasting signal in the SCFR due to the ligation and retention of SCFR at the expressing cell surface. Mature rat SCF is a 248 amino acid (aa) type I transmembrane (TM) glycoprotein (aa 26-273). It contains a 189 aa extracellular region (aa 26-214) plus a 36 aa cytoplasmic domain (aa 238-273). Proteolytic cleavage after Ala190 generates a 25-38 kDa soluble form that circulates as both a monomer and noncovalent homodimer. There is also possible cleavage after Phe183 that will create a similarly-sized isoform. An alternative splice form also exists that contains a Gly substitution for aa 174-292. This 28-32 kDa TM form may also undergo cleavage to generate a soluble 19-21 kDa isoform. Over aa 26-189, rat SCF shares 94%, 77% and 79% aa sequence identity with mouse, feline and human SCF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KITLG
• Uniprot:
  • P21581()