SCF/c-kit Ligand Antibody (40215) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: B/N
• Clone: 40215
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

SCF/c-kit Ligand Antibody (40215) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant mouse SCF/c-kit Ligand
  Lys26-Ala189
  Accession # P20826
• Specificity: Detects mouse SCF/c-kit Ligand in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human SCF.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: KITLG
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody (40215) [Alexa Fluor® 594]

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72-75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KITLG