SART1 Antibody (865709)

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and EL-4 mouse lymphoblast cell line. Gels were loaded with 20 µg of HepG2 and 30 µg of EL-4 cytoplasmic (Cyto) extracts and 10 µg of HepG2 and 15 µg of EL-4 nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SART1 Monoclonal Antibody (Catalog # MAB8034) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SART1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SART1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human SART1 Monoclonal Antibody (Catalog # MAB8034) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 865709
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SART1 Antibody (865709) Summary

• Immunogen: E. coli-derived recombinant human SART1
  Asn647-Lys800
  Accession # O43290
• Specificity: Detects human SART1 in ELISAs and Western Blots. Detects mouse SART1 in Western Blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SART1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SART1 Antibody (865709)

• Allergen Hom s 1
• Ara1
• HAF
• HOMS1
• hSART-1
• hSnu66
• hypoxia-associated factor
• IgE Autoantigen
• MGC2038
• SART1
• SART-1
• SART1(259) protein
• SART1(800) protein
• SART1259
• small nuclear ribonucleoprotein 110kDa (U4/U6.U5)
• SNRNP110
• SNU66 homolog
• Snu66
• squamous cell carcinoma antigen recognised by T cells
• Squamous cell carcinoma antigen recognized by T cells 1
• squamous cell carcinoma antigen recognized by T cells
• U4/U6.U5 tri-snRNP-associated 110 kDa protein
• U4/U6.U5 tri-snRNP-associated protein 1

Background

Hypoxia-associated factor (HAF), also known as SART1, is a widely expressed 125 kDa intracellular protein that promotes assembly of the U2-type pre-mRNA spliceosome. SART1 is also an E3 ubiquitin ligase that promotes the ubiquitination and degradation of HIF-1 alpha. In contrast, it enhances the transcriptional activity of HIF-2 alpha. SART1 is upregulated in many tumor types and enables tumor growth during chronic hypoxia. Within amino acids 647-800, human SART1 shares 100% aa sequence identity with mouse and rat SART1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SART1
• Uniprot:
  • O43290()