SART1 Antibody (865709) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 865709
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

SART1 Antibody (865709) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human SART1
  Asn647-Lys800
  Accession # O43290
• Specificity: Detects human SART1 in ELISAs and Western Blots. Detects mouse SART1 in Western Blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SART1 Antibody (865709) [Alexa Fluor® 594]

• Allergen Hom s 1
• Ara1
• HAF
• HOMS1
• hSART-1
• hSnu66
• hypoxia-associated factor
• IgE Autoantigen
• MGC2038
• SART1
• SART-1
• SART1(259) protein
• SART1(800) protein
• SART1259
• small nuclear ribonucleoprotein 110kDa (U4/U6.U5)
• SNRNP110
• SNU66 homolog
• Snu66
• squamous cell carcinoma antigen recognised by T cells
• Squamous cell carcinoma antigen recognized by T cells 1
• squamous cell carcinoma antigen recognized by T cells
• U4/U6.U5 tri-snRNP-associated 110 kDa protein
• U4/U6.U5 tri-snRNP-associated protein 1

Background

Hypoxia-associated factor (HAF), also known as SART1, is a widely expressed 125 kDa intracellular protein that promotes assembly of the U2-type pre-mRNA spliceosome. SART1 is also an E3 ubiquitin ligase that promotes the ubiquitination and degradation of HIF-1 alpha. In contrast, it enhances the transcriptional activity of HIF-2 alpha. SART1 is upregulated in many tumor types and enables tumor growth during chronic hypoxia. Within amino acids 647-800, human SART1 shares 100% aa sequence identity with mouse and rat SART1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.