SAPS3 Overexpression Lysate (Native)

Images

 
Western Blot: SAPS3 Lysate [NBL1-15690] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for SAPS3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SAPS3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC214636 and based on accession number NM_018312. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SAPS domain family, member 3 (SAPS3), mRNA.
Gene
PPP6R3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
88.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SAPS3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SAPS3 Overexpression Lysate (Native)

  • C11orf23SAPLa
  • chromosome 11 open reading frame 23
  • DKFZp781E17107
  • DKFZp781E2374
  • DKFZp781O2362
  • FLJ11058
  • FLJ43065
  • KIAA1558MGC125711
  • PP6R3MGC125712
  • protein phosphatase 6, regulatory subunit 3
  • SAPLSAP190
  • SAPS domain family member 3
  • SAPS3SAPS domain family, member 3
  • serine/threonine-protein phosphatase 6 regulatory subunit 3
  • Sporulation-induced transcript 4-associated protein SAPL

Background

Protein phosphatase regulatory subunits, such as SAPS3, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS3 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 300141) (Stefansson and Brautigan, 2006 [PubMed 16769727]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19048
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-47289
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2883
Species: Hu, Mu
Applications: WB, IP
NBP1-80898
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-48803
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-55256
Species: Hu
Applications: WB, IHC-P, IP
NBP1-88014
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00004068-M01
Species: Hu
Applications: WB, ELISA
AF2558
Species: Hu, Mu
Applications: WB, Simple Western
NB100-1886
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-384
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NBP2-14253
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-76788
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-52414
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, ICC, IF
NBP2-24449
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow
NBP2-22128
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P
AF4300
Species: Hu
Applications: IHC
MAB5074
Species: Hu
Applications: WB
NBP1-30646
Species: Hu
Applications: WB, IHC, IHC-P
NBL1-15690
Species: Hu
Applications: WB

Publications for SAPS3 Lysate (NBL1-15690) (0)

There are no publications for SAPS3 Lysate (NBL1-15690).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SAPS3 Lysate (NBL1-15690) (0)

There are no reviews for SAPS3 Lysate (NBL1-15690). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SAPS3 Lysate (NBL1-15690) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SAPS3 Products

Bioinformatics Tool for SAPS3 Lysate (NBL1-15690)

Discover related pathways, diseases and genes to SAPS3 Lysate (NBL1-15690). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SAPS3 Lysate (NBL1-15690)

Discover more about diseases related to SAPS3 Lysate (NBL1-15690).
 

Pathways for SAPS3 Lysate (NBL1-15690)

View related products by pathway.

Blogs on SAPS3

There are no specific blogs for SAPS3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SAPS3 Antibody
NBP2-34049

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SAPS3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PPP6R3