Western Blot: SAP30L Antibody [NB100-491] - Analysis of SAP30L using NB100-491 on GST-hSAP30L fusion protein in NIH3T3 cells (Lane 1), wild-type transfected hSAP30L and endogenous hSAP30L in HEK293 cells, (Lane 2), and ...read more
This antibody has been tested in Western blot. No other applications have been tested. Recommended dilution below. Western Blot - 1:500 to 1:1,000* *However, the investigator should determine the optimal dilution. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kDa and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22 kDa. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or post-translational modification.
This antibody reacts with wild-type transfected and endogenous human SAP30L protein. This antibody does not react with endogenous non-human SAP30L.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for SAP30L Antibody
HCV non-structural protein 4A-transactivated protein 2
histone deacetylase complex subunit SAP30L
Sin3 corepressor complex subunit SAP30L
Sin3A associated protein p30-like
Sin3-associated protein p30-like
A novel TGF- beta up-regulated mRNA species, the Sin3-associated protein 30-like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30 it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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