SAP30L Antibody - BSA Free

Western Blot: SAP30L Antibody [NB100-491] - Analysis of SAP30L using NB100-491 on GST-hSAP30L fusion protein in NIH3T3 cells (Lane 1), wild-type transfected hSAP30L and endogenous hSAP30L in HEK293 cells, (Lane 2), and endogenous hSAP30L in non-transfected MCF-7 cells.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SAP30L Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SAP30L Antibody - BSA Free (NB100-491) is a polyclonal antibody validated for use in WB. Anti-SAP30L Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to residues 165-183 (VKSNKSRLDQKSEGGKQLE) of human SAP30L.
• Specificity: This is specific for SAP30L.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAP30L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: This antibody has been tested in Western blot. No other applications have been tested. Recommended dilution below. Western Blot - 1:500 to 1:1,000* *However, the investigator should determine the optimal dilution. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kDa and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22 kDa. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or post-translational modification.
• Control:

  293 Whole Cell Lysate
  MCF-7 Whole Cell Lysate

Reactivity Notes

This antibody reacts with wild-type transfected and endogenous human SAP30L protein. This antibody does not react with endogenous non-human SAP30L.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SAP30L Antibody - BSA Free

• DKFZp667L2214
• FLJ11526
• FLJ36497
• HCV non-structural protein 4A-transactivated protein 2
• histone deacetylase complex subunit SAP30L
• NS4ATP2FLJ23595
• SAP30-like
• Sin3 corepressor complex subunit SAP30L
• Sin3A associated protein p30-like
• Sin3-associated protein p30-like

Background

A novel TGF- beta up-regulated mRNA species, the Sin3-associated protein 30-like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30 it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAP30L Antibody (NB100-491)(1)

Publication using NB100-491

• Bennett GA, Palliser HK, Shaw JC et al. Prenatal Stress Alters Hippocampal Neuroglia and Increases Anxiety in Childhood. Dev. Neurosci. 2015-08-13 [PMID: 26279160] (Human)

Bioinformatics

• Gene Symbol: SAP30L