SAP130 Antibody (JG63-53)

Western Blot: SAP130 Antibody (JG63-53) [NBP2-76869] - Western blot analysis of SF3B3 on Jurkat and Hela cells lysates using anti-SF3B3 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: SAP130 Antibody (JG63-53) [NBP2-76869] - ICC staining SF3B3 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.

Flow Cytometry: SAP130 Antibody (JG63-53) [NBP2-76869] - Flow cytometric analysis of LOVO cells with SF3B3 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.

Product Discontinued

SAP130 Antibody (JG63-53) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SAP130 aa 1061-1217 / 1217. (SwissProt: Q15393 Human; SwissProt: Q921M3 Mouse; Entrez Gene: 292019 Rat)
• Localization: Nucleus. Spliceosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SF3B3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 136 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SAP130 Antibody (JG63-53)

• KIAA0017splicing factor 3b, subunit 3, 130kD
• Pre-mRNA-splicing factor SF3b 130 kDa subunit
• RSE1
• SAP130SAP 130
• SF3B130
• SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit
• Spliceosome-associated protein 130
• splicing factor 3B subunit 3
• splicing factor 3b, subunit 3, 130kDa
• STAF130

Background

SAP130 encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SF3B3