SAP102 Antibody (S19-2)

Western Blot: SAP102 Antibody (S19-2) [NBP1-21672] - Rat brain extract

Immunocytochemistry/Immunofluorescence: SAP102 Antibody (S19-2) [NBP1-21672] - analysis using Mouse Anti-SAP102 Monoclonal Antibody, Clone S19-2 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SAP102 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X.

Product Discontinued

SAP102 Antibody (S19-2) Summary

• Immunogen: Fusion protein amino acids 1-120 of rat SAP102
• Localization: Basolateral Cell Membrane , Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Growth Cone
• Specificity: Detects approx 105kDa. No cross-reactivity against PSD95, SAP97 or Chapsyn-110.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLG3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:1000
• Application Notes: 1 ug/ml was sufficient for detection of Sap102 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SAP102 Antibody (S19-2)

• discs, large homolog 3 (Drosophila)
• KIAA1232discs, large homolog 3 (neuroendocrine-dlg, Drosophila)
• MRX90MRX
• NEDLGdisks large homolog 3
• neuroendocrine-DLG
• SAP-102
• SAP102NE-Dlg
• Synapse-associated protein 102
• XLMR

Background

Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homolgy 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with the carboxy-terminal domain of the N-methyl-Daspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras- GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAP102 Antibody (NBP1-21672)(1)

Publication using NBP1-21672

• Lecointre M, Vezier C, Benard M et al. Age-dependent alterations of the NMDA receptor developmental profile and adult behavior in postnatally ketamine-treated mice. Dev Neurobiol. 2014-09-13 [PMID: 25220981]

Bioinformatics

• Gene Symbol: DLG3
• Entrez:
  • 1741(Human)
  • 53310(Mouse)
  • 58948(Rat)
• Uniprot:
  • Q62936(Rat)