SAA Antibody (Reu86.1)

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: Reu86.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

SAA Antibody (Reu86.1) Summary

• Description: Novus Biologicals Mouse SAA Antibody (Reu86.1) (NB600-1418) is a monoclonal antibody validated for use in IHC and WB. Anti-SAA Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human SAA
• Localization: Secreted
• Specificity: SAA (Reu86.1)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:100
  • Immunohistochemistry-Paraffin 1:10-1:100
  • Western Blot 1:100-1:2000
• Application Notes: Immunohistochemistry paraffin and frozen: Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS containing 0.1% BSA.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for SAA Antibody (Reu86.1)

• SAA
• SAA1
• SAA4
• serum amyloid A1 cluster

Background

The serum amyloid A (SAA) family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma, and constitutive SAAs (C-SAAs). Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000-fold during inflammation. Several studies have stressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging. Expression studies show production of SAA proteins in histologically normal, atherosclerotic, Alzheimer, inflammatory, and tumor tissues. SAA has been found to have binding sites for high density lipoproteins, calcium, laminin, and heparin/heparan-sulfate. Also adhesion motifs were identified and new functions, affecting cell adhesion, migration, proliferation and aggregation discovered. These findings emphasize the importance of SAA in various physiological and pathological processes, including inflammation, atherosclerosis, thrombosis, AA-amyloidosis, rheumatoid arthritis, and neoplasia. SAA has also a number of immunomodulatory roles, it can induce chemotaxis and adhesion molecule expression, has cytokine-like properties and can promote the upregulation of metalloproteinases. It enhances the binding of high-density lipoprotein to macrophages and thus helps in the delivery of lipids to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease processes. In addition, recent experiments suggest that SAA may play a "housekeeping" role in normal human tissues. Elevated levels of SAA over time predispose to secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissue and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid arthritis. The antibody is raised against human SAA and Helix Pomatia Haemocyanine. It reacts specifically with SAA-1, the major isoform of SAA in plasma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SAA Antibody (NB600-1418)(1)

Publication using NB600-1418

• Huppertz C, Jager B, Wieczorek G et al. The NLRP3 inflammasome pathway is activated in sarcoidosis and involved in granuloma formation Eur. Respir. J. 2020-01-16 [PMID: 31949113] (IHC-P)

Bioinformatics

• Gene Symbol: SAA