S1P1/EDG-1 Antibody (MM0045-21L9) [Alexa Fluor® 488]

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Flow Cytometry: S1P1/EDG-1 Antibody (MM0045-21L9) [Alexa Fluor (R) 488] [NB110-93513AF488] - analysis of S1P1 in white blood cells from human blood using anti-S1P1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
MM0045-21L9
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

S1P1/EDG-1 Antibody (MM0045-21L9) [Alexa Fluor® 488] Summary

Immunogen
human recombinant EDG-1 protein
Specificity
This recognizes human EDG-1 in western blot.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
S1PR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Reviewed Applications
Read 1 Review rated 4
using
NB110-93513AF488 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for S1P1/EDG-1 Antibody (MM0045-21L9) [Alexa Fluor® 488]

  • CD363 antigen
  • CD363
  • CHEDG1
  • D1S3362
  • EDG1
  • EDG-1
  • EDG1edg-1
  • Endothelial differentiation G-protein coupled receptor 1
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
  • FLJ58121
  • S1P receptor 1
  • S1P receptor Edg-1
  • S1P1
  • S1P1ECGF1
  • S1PR1
  • sphingosine 1-phosphate receptor 1
  • sphingosine 1-phosphate receptor EDG1
  • Sphingosine 1-phosphate receptor Edg-1
  • sphingosine-1-phosphate receptor 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S1P1/EDG-1 Antibody (NB110-93513AF488) (0)

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Review for S1P1/EDG-1 Antibody (NB110-93513AF488) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NB110-93513AF488:
Filter by Applications
Flow
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry S1P1/EDG-1 NB110-93513AF488
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reviewed by:
Gleb Mironov
Flow Human 04/15/2015
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Summary

ApplicationFlow Cytometry
Sample TestedWhite blood cells from human blood
SpeciesHuman
Lot2014070203-af488

Blocking

Blocking Details10% FBS for 15 min, 37C

Primary Anitbody

Dilution Ratio5 ul of Ab per 100 ul of sample, incubation 45 min, room temperature, DPBS buffer with Ca and Mg

Details

Detection NotesBeckam Gallios flow cytometer
Fixation DetailsCells were fixed after staining 1% formaldehyde, room temperature, DPBS buffer with Ca and Mg

Comments

CommentsIt's good to read in advance which cells express S1PR1

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for S1P1/EDG-1 Antibody (NB110-93513AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NB110-93513AF647
Alexa Fluor 700 Labeled NB110-93513AF700

Additional S1P1/EDG-1 Products

Bioinformatics Tool for S1P1/EDG-1 Antibody (NB110-93513AF488)

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Recent Reviews

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Gleb Mironov
04/15/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol S1PR1
Entrez