S100A4 Antibody

Western Blot: S100A4 Antibody [NB100-55404] - (0.05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: S100A4 Antibody [NB100-55404] - (2ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

S100A4 Antibody Summary

• Description: Novus Biologicals Goat S100A4 Antibody (NB100-55404) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-S100A4 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CNEFFEGFPDKQP corresponding to C-Terminus according to NP_062427.1.
• Localization: Nuclear
• Specificity: Both variants (NP_002952.1; NP_062427.1) represent identical protein.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: S100A4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.05-0.2 ug/ml
• Application Notes: WB: Approx. 13 kDa band observed in human placenta lysates (calculated MW of 11.7 kDa band according to NP_002952.1and NP_062427.1). IHC-P: Human small intestine shows strong nuclear staining in some cells and weaker cytoplasm staining in some other cells. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 31024074).
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  S100A4 Overexpression Lysate
  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31024074).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for S100A4 Antibody

• 18A2
• 42A
• Calvasculin
• CAPL
• CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
• fibroblast-specific protein-1,42A
• FSP1
• leukemia multidrug resistance associated protein
• malignant transformation suppression 1
• Metastasin
• MTS1
• murine placental homolog)
• P9KA
• PEL98
• Placental calcium-binding protein
• Protein Mts1
• protein S100-A4
• S100 calcium binding protein A4
• S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
• S100 calcium-binding protein A4
• S100A4

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A4 Antibody (NB100-55404)(4)

Publications using NB100-55404

• Ye Y, Xu L, Ding H et al. Pyruvate kinase M2 mediates fibroblast proliferation to promote tubular epithelial cell survival in acute kidney injury FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-07-01 [PMID: 34160104]
• Gui, Y;Lu, Q;Gu, M;Wang, M;Liang, Y;Zhu, X;Xue, X;Sun, X;He, W;Yang, J;Zhao, AZ;Xiao, B;Dai, C; Fibroblast mTOR/PPAR gamma/HGF axis protects against tubular cell death and acute kidney injury Cell Death Differ. 2019-04-25 [PMID: 31024074] (ICC/IF, Mouse)
• Jiang L, Xu L, Mao J et al. Rheb/mTORC1 Signaling Promotes Kidney Fibroblast Activation and Fibrosis. J Am Soc Nephrol 2013-05-09 [PMID: 23661807]
• Li ZH, Bresnick AR. The S100A4 metastasis factor regulates cellular motility via a direct interaction with myosin-IIA. Cancer Res 2006-05-15 [PMID: 16707441]

Bioinformatics

• Gene Symbol: S100A4
• Entrez:
  • 6275(Human)
  • 20198(Mouse)
  • 24615(Rat)
• Uniprot:
  • P26447()