S100A4 Antibody (SD200-08)

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (SD200-08) [NBP2-67740] - Staining S100A4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: S100A4 Antibody (SD200-08) [NBP2-67740] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-S100A4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: S100A4 Antibody (SD200-08) [NBP2-67740] - Analysis of Jurkat cells with S100A4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (SD200-08) [NBP2-67740] - Staining S100A4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (SD200-08) [NBP2-67740] - Staining S100A4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: S100A4 Antibody (SD200-08) [NBP2-67740] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100A4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: S100A4 Antibody (SD200-08) [NBP2-67740] - Analysis of paraffin-embedded human lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: S100A4 Antibody (SD200-08) [NBP2-67740] - Analysis of paraffin-embedded human tonsil tissue using anti-S100A4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: S100A4 Antibody (SD200-08) [NBP2-67740] - Analysis of paraffin-embedded mouse lung tissue using anti-S100A4 antibody. Counter stained with hematoxylin.

Product Discontinued

S100A4 Antibody (SD200-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human S100A4 aa 1-101 / 101. (SwissProt: P26447 Human; SwissProt: P07091 Mouse; SwissProt: P05942 Rat)
• Localization: Extracellular space, nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: S100A4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for S100A4 Antibody (SD200-08)

• 18A2
• 42A
• Calvasculin
• CAPL
• CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
• fibroblast-specific protein-1,42A
• FSP1
• leukemia multidrug resistance associated protein
• malignant transformation suppression 1
• Metastasin
• MTS1
• murine placental homolog)
• P9KA
• PEL98
• Placental calcium-binding protein
• Protein Mts1
• protein S100-A4
• S100 calcium binding protein A4
• S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
• S100 calcium-binding protein A4
• S100A4

Background

S100A4 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100A4