S100A4 Antibody (1092141) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 1092141
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

S100A4 Antibody (1092141) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived mouse S100A4
  Ala2-Lys101
  Accession # P07091
• Specificity: Detects recombinant mouse S100A4 protein in Direct ELISA
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A4 Antibody (1092141) [Alexa Fluor® 532]

• 18A2
• 42A
• Calvasculin
• CAPL
• CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
• fibroblast-specific protein-1,42A
• FSP1
• leukemia multidrug resistance associated protein
• malignant transformation suppression 1
• Metastasin
• MTS1
• murine placental homolog)
• P9KA
• PEL98
• Placental calcium-binding protein
• Protein Mts1
• protein S100-A4
• S100 calcium binding protein A4
• S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
• S100 calcium-binding protein A4
• S100A4

Background

S100A4 (also named Metastasin, Mtsl and Calvasculin) is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of proteins (1‑5). S100 family members belong to the EF-hand superfamily of Ca⁺⁺-binding proteins. These participate in both calcium‑dependent and calcium‑independent protein‑protein interactions. The hallmark of this superfamily is the EF-hand motif that consists of a Ca⁺⁺-binding site flanked by two alpha -helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium‑binding protein (6). Mouse S100A4 is 101 amino acids (aa) in length (1, 2). It contains two EF hand domains (aa 12‑47 and aa 50‑85). The first domain has a 14 aa cation-binding motif and binds Ca⁺⁺ with low affinity. The second Ca⁺⁺-binding motif is 12 aa in length and binds Ca⁺⁺ with high affinity. S100A4 has no classical signal sequence but is secreted from cells (3, 7). Mouse S100A4 shares 93%, 96% and 89% aa identity with human, rat and canine S100A4, respectively. S100A4 exists as dimer (8, 9, 10). Extracellular S100A4 is reported to induce MMP production, activate MMPs, promote neurite outgrowth and stimulate cardiomyocyte proliferation (4, 10, 11, 12, 13). Within the cell, dimers are likely the functional unit. Here, they are constitutive homo‑ or heterodimers (with S100A1) that interact with Ca⁺⁺, undergo a conformational change, and subsequently bind to cytoplasmic targets. Known targets include p53, Myosin heavy chain II, F-actin and Liprin beta 1 (4, 14). In general, it can be said that S100A4 blocks target phosphorylation and multimerization (4, 7, 14). S100A4 activity has been associated with cell transformation. It seems likely this is either coincidental, or a consequence, rather than a cause of transformation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.