S100A11 Antibody (2F4) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: S100A11 Antibody (2F4) [H00006282-M01] - Analysis of monoclonal antibody to S100A11 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: S100A11 Antibody (2F4) [H00006282-M01] - Analysis of monoclonal antibody to S100A11 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

Sandwich ELISA: S100A11 Antibody (2F4) [H00006282-M01] - Detection limit for recombinant GST tagged S100A11 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

S100A11 Antibody (2F4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: S100A11 (AAH14354, 1 a.a. ~ 105 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAKISSPTETERCIESLIAVFQKYAGKDGYNYTLSKTEFLSFMNTELAAFTKNQKDPGVLDRMMKKLDTNSDGQLDFSEFLNLIGGLAMACHDSFLKAVPSQKRT
• Specificity: S100 calcium binding protein A11 (calgizzarin)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100A11
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for S100A11 Antibody (2F4) - Azide and BSA Free

• Calgizzarin
• Metastatic lymph node gene 70 protein
• MLN 70
• MLN70
• protein S100-A11
• Protein S100-C
• S100 calcium binding protein A11
• S100 calcium-binding protein A11 (calgizzarin)
• S100 calcium-binding protein A11
• S100A11
• S100C
• S100CS100 calcium binding protein A11 (calgizzarin)

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A11 Antibody (H00006282-M01)(2)

Publications using H00006282-M01

• Zhu L, Okano S, Takahara M et al. Expression of S100 protein family members in normal skin and sweat gland tumors. J Dermatol Sci. 2013-06-01 [PMID: 23623205] (IHC-P, Human)
  
  Details:
  Psoriasin/HID5/S100A7 Clone 47C1068 (IMG-409A), IHC (paraffin): Normal human skin and sweat gland tumors, Figs 1k-O & 5A-G, Tables 1-3. Note: The 47C1068 S100A7 mAb was used at 10 ug/ml.
• Li J, Riau AK, Setiawan M et al. S100A expression in normal corneal-limbal epithelial cells and ocular surface squamous cell carcinoma tissue. Mol Vis. 2011-08-20 [PMID: 21897749]

Bioinformatics

• Gene Symbol: S100A11
• Entrez:
  • 6282(Human)
• OMIM:
  • 603114(Human)
• Uniprot:
  • AAH14354(Human)