Ryk Antibody [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Ryk Antibody [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Ryk
  Arg35-Arg211
  Accession # Q01887
• Specificity: Detects mouse and human Ryk in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ryk Antibody [Alexa Fluor® 350]

• D3S3195
• EC 2.7.10
• EC 2.7.10.1
• hydroxyaryl-protein kinase
• JTK5
• JTK5A protein tyrosine kinase
• JTK5A
• RYK receptor-like tyrosine kinase
• Ryk
• RYK1
• tyrosine-protein kinase RYK

Background

Ryk (related to tyrosine (Y) kinase; also VIK and MRK) is a member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Mouse Ryk is 594 amino acids (aa) in length. It contains a 177 aa extracellular region (aa 35‑211) that shows one WIF-1-like domain (aa 51‑180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318‑581). There is one alternate start site at Met117. Over aa 35‑211, mouse Ryk is 94% aa identical to human Ryk, and shows absolute (100%) aa identity to rat Ryk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.