Ryk Antibody (484804) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 484804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Ryk Antibody (484804) [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Ryk
  Met1-Arg224
  Accession # P34925
• Specificity: Detects human Ryk in ELISA.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ryk Antibody (484804) [Alexa Fluor® 532]

• D3S3195
• EC 2.7.10
• EC 2.7.10.1
• hydroxyaryl-protein kinase
• JTK5
• JTK5A protein tyrosine kinase
• JTK5A
• RYK receptor-like tyrosine kinase
• Ryk
• RYK1
• tyrosine-protein kinase RYK

Background

Ryk (related to tyrosine (Y) kinase; also H-RYK) is a 70-90 kDa, atypical member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt, and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Human Ryk is 604 amino acids (aa) in length. It contains a 25 aa signal sequence and a 199 aa extracellular region (aa 26-224) that shows one WIF-1-like domain (aa 63‑191). The cytoplasmic region possesses a nonfunctional Ser/Thr protein kinase domain (aa 327-600). One isoform exists that shows a 31 aa substitution between aa’s 18-46. Over aa 47-224, human Ryk is 94% aa identical to mouse Ryk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.