Ryanodine Receptor 1 Antibody (C3-33) [Alexa Fluor™ Plus 680]

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, Fi, Gp, Rb
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: C3-33
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

Ryanodine Receptor 1 Antibody (C3-33) [Alexa Fluor™ Plus 680] Summary

• Immunogen: Canine cardiac ryanodine receptor (RyR2)
• Localization: Membrane; Multi-pass membrane protein
• Specificity: Detects ryanodine receptor 2 (RyR 2) and weakly detects RyR 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RYR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 12456488). Bovine (PMID: 15896674).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Ryanodine Receptor 1 Antibody (C3-33) [Alexa Fluor™ Plus 680]

• CCO
• central core disease of muscle
• MHS
• MHS1
• PPP1R137
• protein phosphatase 1, regulatory subunit 137
• ryanodine receptor 1 (skeletal)
• Ryanodine Receptor 1
• ryanodine receptor type1
• RYDR
• RYR
• RyR1
• RYR-1
• sarcoplasmic reticulum calcium release channel
• Skeletal muscle calcium release channel
• skeletal muscle ryanodine receptor
• Skeletal muscle-type ryanodine receptor
• SKRR
• type 1-like ryanodine receptor

Background

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RYR1