Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms. In chickens, this detects the alpha, beta and cardiac isoforms. This detects RyR-3 in mouse cells. In frog, this detects the alpha and beta isoforms. In fish, this detects the alpha isoform.
Test in a species/application not listed above to receive a full credit towards a future purchase.
This antibody can be used in Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, and Western Blot. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain results in intense staining of cerebral Purkinje neurons. Recommended Starting Dilutions*: IHC-Fr: 1/1000 WB: 1/5000 Not tested in other applications. *Optimal dilutions should be determined by the end user. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
565 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for Ryanodine Receptor 1 Antibody (34C)
central core disease of muscle
ryanodine receptor 1 (skeletal)
ryanodine receptor 1
ryanodine receptor type1
sarcoplasmic reticulum calcium release channel
Skeletal muscle calcium release channel
skeletal muscle ryanodine receptor
Skeletal muscle-type ryanodine receptor
type 1-like ryanodine receptor
The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.