Recombinant Human RWDD1 His Protein Summary
| Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-243 of Human RWDD1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTDYGEE QRNELEALES IYPDSFTVLS ENPPSFTITV TSEAGENDET VQTTLKFTYS EKYPDEAPLY EIFSQENLED NDVSDILKLL ALQAEENLGM VMIFTLVTAV QEKLNEIVDQ IKTRREEEKK QKEKEAEEAE KQLFHGTPVT IENFLNWKAK FDAELLEIKK KRMKEEEQAG KNKLSGKQLF ETDHNLDTSD IQFLEDAGNN VEVDESLFQE MDDLELEDDE DDPDYNPADP ESDSAD |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
RWDD1 |
| Purity |
>85%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
30.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl,10% glycerol, 2 mM DTT |
| Preservative |
No Preservative |
| Concentration |
0.5 mg/ml |
| Purity |
>85%, by SDS-PAGE |
Alternate Names for Recombinant Human RWDD1 His Protein
Background
RWDD1, also known as CGI-24 or PTD013, belongs to the RWDD1/GIR2 family. This protein protects DRG2 (developmentally regulated GTP binding protein 2), from proteolytic degradation. RWDD1 is a novel RWD domain containing protein. RWD domain refers to three signature motifs in proteins: RING finger, WD-repeats, and yeast DEAD (DEXD)-like motif. The function of this domain is not clear enough. Present evidence suggests that RWD domain might be necessary for protein-protein interaction. Recombinant human RWDD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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