KPNA5 Antibody (1D2)

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - Analysis of KPNA5 expression in transfected 293T cell line by KPNA5 monoclonal antibody (M01), clone 1D2.Lane 1: KPNA5 transfected lysate(60.7 KDa).Lane 2: Non-transfected lysate.

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - Analysis of KPNA5 over-expressed 293 cell line, cotransfected with KPNA5 Validated Chimera RNAi ( Cat # H00003841-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KPNA5 monoclonal antibody (M01), clone 1D2 (Cat # H00003841-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - KPNA5 monoclonal antibody (M01), clone 1D2 Analysis of KPNA5 expression in HepG2.

ELISA: KPNA5 Antibody (1D2) [H00003841-M01] - Detection limit for recombinant GST tagged KPNA5 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP, KD
• Clone: 1D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

KPNA5 Antibody (1D2) Summary

• Immunogen: KPNA5 (AAH47409.1, 1 a.a. ~ 539 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKRRNVYLPRNDESMLESPIQDPDISSTVPIPEEEVVTTDMVQMIFSNNADQQLTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCTLQFEAAWALTNIASGTFLHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEILPPLLELLTNSNRLTTTRNAVWALSNLCRGKNPPPNFSKVSPCLNVLSRLLFSSDPDVLADVCWALSYLSDGPNDKIQAVIDSGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALPCLLHLLSSPKESIRKEACWTVSNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQIRYLVALGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQESKQNGIGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGVEEDDPSIVPQVDENQQQFIFQQQEAPMDGFQL
• Localization: Cytoplasmic
• Specificity: KPNA5 - karyopherin alpha 5 (importin alpha 6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KPNA5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA. Use in immunoprecipitation reported in scientific literature (PMID: 26052702).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KPNA5 Antibody (1D2)

• importin alpha 6
• importin subunit alpha-6
• IPOA6
• karyopherin alpha 5 (importin alpha 6)
• Karyopherin subunit alpha-5
• SRP6

Background

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KPNA5 Antibody (H00003841-M01)(1)

Publication using H00003841-M01

• Kim NH, Yoshimaru T, Chen YA et al. BIG3 Inhibits the Estrogen-Dependent Nuclear Translocation of PHB2 via Multiple Karyopherin-Alpha Proteins in Breast Cancer Cells. PLoS One 2015-01-01 [PMID: 26052702] (IP, Human)

Bioinformatics

• Gene Symbol: KPNA5
• Entrez:
  • 3841(Human)
• OMIM:
  • 604545(Human)
• Uniprot:
  • AAH47409(Human)