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RUVBL2 Antibody (OTI1A6)

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Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from 9 Human tissue by using anti-RUVBL2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from 9 Human tissue by using anti-RUVBL2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: ...read more
Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2.
Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Human tonsil using anti-RUVBL2 mouse monoclonal antibody.
Flow Cytometry: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RUVBL2 antibody, and then ...read more
Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] Analysis of extracts (35ug) from 9 different cell lines by using anti-RUVBL2 monoclonal antibody.
Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiRUVBL2 monoclonal antibody.(1:200)
Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RUVBL2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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RUVBL2 Antibody (OTI1A6) Summary

Immunogen
Human recombinant protein fragmengt corresponding to amino acids 113-370 of human RUVBL2(NP_006657) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RUVBL2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RUVBL2 Antibody (OTI1A6)

  • 48 kDa TATA box-binding protein-interacting protein
  • 51 kDa erythrocyte cytosolic protein
  • EC 3.6.1
  • EC 3.6.4.12,48 kDa TBP-interacting protein
  • ECP51TIP49B
  • erythrocyte cytosolic protein, 51-KD
  • INO80 complex subunit J
  • INO80JTAP54-beta
  • Repressing pontin 52
  • Reptin 52
  • REPTIN
  • Reptin52
  • RuvB (E coli homolog)-like 2
  • RuvB-like 2 (E. coli)
  • ruvB-like 2
  • RVB2
  • TBP-interacting protein, 48-KD
  • TIH2
  • TIP48ECP-51
  • TIP49b
  • TIP60-associated protein 54-beta

Background

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Paraffin-embedded mouse testis. RUVBL1 antibody diluted at 1:500.
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Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
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Western Blot: TIP60 Antibody [NBP2-24613] - analysis of TIP60 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using this antibody. 25 ug/ml.
Immunohistochemistry-Paraffin: TIP60 Antibody [NBP2-24613] - analysis of TIP60 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Simple Western: TIP60 Antibody [NBP2-24613] - Simple Western lane view shows a specific band for TIP60 in 0.2 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(1 Publication)
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beta-Catenin
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Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).
Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - IHC analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain.
(3 Reviews)
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Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
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Immunoprecipitation: INO80 Antibody [NBP1-78758] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. AAffinity purified rabbit anti-INO80 antibody used for IP at 6 mcg/mg lysate. INO80 was also immunoprecipitated by rabbit anti-INO80 antibody NBP1-78759 which recognizes a downstream epitope.
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beta Amyloid
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Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.
Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).
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Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
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H00008086-M02
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Western Blot: AAAS Antibody (5A1) [H00008086-M02] - Western Blot analysis of AAAS expression in HeLa ( Cat # L013V1 ).
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Immunocytochemistry/Immunofluorescence: CD82/Kai-1 Antibody [NBP1-76775] - Human Colon cells.
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody [NBP1-76775] - Human colon tissue section.
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Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Human Pancreatic carcinoma showing strong staining of tumor cells.
Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.
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Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
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Immunocytochemistry/Immunofluorescence: NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
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FLJ21908 Antibody
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Western Blot: FLJ21908 Antibody [NBP1-89849] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/Immunofluorescence: FLJ21908 Antibody [NBP1-89849] - Staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and cytoskeleton (intermediate filaments).
Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89849] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Species: Hu
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BAF53A Antibody
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Immunocytochemistry/Immunofluorescence: BAF53A Antibody [NB100-61628] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.
Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DA
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SRCAP Antibody
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SRCAP Antibody
(1 Publication)
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PHLDA3 Antibody
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Western Blot: PHLDA3 Antibody [NBP1-80683] - Analysis in control (vector only transfected HEK293T lysate) and PHLDA3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry-Paraffin: PHLDA3 Antibody [NBP1-80683] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
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Western Blot: Pleckstrin Antibody (6H8) [NBP2-02300] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Pleckstrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Pleckstrin.
Immunohistochemistry-Paraffin: Pleckstrin Antibody (6H8) [NBP2-02300] Staining of paraffin-embedded prostate tissue using anti-Pleckstrin mouse monoclonal antibody.
Flow Cytometry: Pleckstrin Antibody (6H8) [NBP2-02300] - HEK293T cells transfected with either pCMV6-ENTRY Pleckstrin.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Pleckstrin mouse monoclonal, and then analyzed by flow cytometry.
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Applications: WB, Flow, IHC, IHC-P
TATA binding
protein TBP Antibody
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Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Staining of Pig Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - NBP2-12933 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Analysis of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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Antibody
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Western blot shows lysates of Raji human Burkitt's lymphoma cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human Miz‑1/ZBTB17 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3760) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Miz-1/ZBTB17 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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Antibody (WA-1 (HJ21)
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Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(11 Publications)
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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

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Bioinformatics

Gene Symbol RUVBL2
Entrez