RUVBL2 Antibody (OTI1A6)

Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from 9 Human tissue by using anti-RUVBL2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2.

Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Human tonsil using anti-RUVBL2 mouse monoclonal antibody.

Flow Cytometry: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RUVBL2 antibody, and then analyzed by flow cytometry.

Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2.

Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] Analysis of extracts (35ug) from 9 different cell lines by using anti-RUVBL2 monoclonal antibody.

Western Blot: RUVBL2 Antibody (1A6) [NBP2-01764] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiRUVBL2 monoclonal antibody.(1:200)

Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RUVBL2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RUVBL2 Antibody (1A6) [NBP2-01764] - Staining of paraffin-embedded Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.6 mg/ml

RUVBL2 Antibody (OTI1A6) Summary

• Immunogen: Human recombinant protein fragmengt corresponding to amino acids 113-370 of human RUVBL2(NP_006657) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RUVBL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.6 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RUVBL2 Antibody (OTI1A6)

• 48 kDa TATA box-binding protein-interacting protein
• 51 kDa erythrocyte cytosolic protein
• EC 3.6.1
• EC 3.6.4.12,48 kDa TBP-interacting protein
• ECP51TIP49B
• erythrocyte cytosolic protein, 51-KD
• INO80 complex subunit J
• INO80JTAP54-beta
• Repressing pontin 52
• Reptin 52
• REPTIN
• Reptin52
• RuvB (E coli homolog)-like 2
• RuvB-like 2 (E. coli)
• ruvB-like 2
• RVB2
• TBP-interacting protein, 48-KD
• TIH2
• TIP48ECP-51
• TIP49b
• TIP60-associated protein 54-beta

Background

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RUVBL2 Antibody (NBP2-01764)(2)

Publications using NBP2-01764

• Curreli S, Benedetti F, Yuan W et al. Characterization of the interactome profiling of Mycoplasma fermentans DnaK in cancer cells reveals interference with key cellular pathways Frontiers in Microbiology 2022-10-28 [PMID: 36386669] (Western Blot, Block/Neutralize)
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: RUVBL2
• Entrez:
  • 10856(Human)