RUVBL2 Antibody (A1E12-R)

Western Blot: RUVBL2 Antibody (A1E12-R) [NBP3-32922] - Western blot analysis of RUVBL2 on different lysates with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Daudi cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: SK-Br-3 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: RAW264.7 cell lysate (20 ug/Lane) Lane 8: C6 cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (20 ug/Lane) Lane 11: Mouse testis tissue lysate (40 ug/Lane) Lane 12: Rat testis tissue lysate (40 ug/Lane) Lane 13: Mouse thymus tissue lysate (40 ug/Lane) Lane 14: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32922) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: RUVBL2 Antibody (A1E12-R) [NBP3-32922] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32922) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody (A1E12-R) [NBP3-32922] - Immunocytochemistry analysis of HeLa cells labeling RUVBL2 with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-RUVBL2 antibody (NBP3-32922) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clone: A1E12-R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RUVBL2 Antibody (A1E12-R) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human RUVBL2 aa 130-321 / 463. (Uniprot: Q9Y230)
• Localization: Nucleus matrix, nucleoplasm, cytoplasm, membrane.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RUVBL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RUVBL2 Antibody (A1E12-R)

• 48 kDa TATA box-binding protein-interacting protein
• 51 kDa erythrocyte cytosolic protein
• EC 3.6.1
• EC 3.6.4.12,48 kDa TBP-interacting protein
• ECP51TIP49B
• erythrocyte cytosolic protein, 51-KD
• INO80 complex subunit J
• INO80JTAP54-beta
• Repressing pontin 52
• Reptin 52
• REPTIN
• Reptin52
• RuvB (E coli homolog)-like 2
• RuvB-like 2 (E. coli)
• ruvB-like 2
• RVB2
• TBP-interacting protein, 48-KD
• TIH2
• TIP48ECP-51
• TIP49b
• TIP60-associated protein 54-beta

Background

RUVBL2 encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RUVBL2