RUNX1T1/ETO Antibody (5A12) - Azide and BSA Free

Western Blot: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.Lane 1: RUNX1T1 transfected lysate(67.566 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Analysis of monoclonal antibody to RUNX1T1 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Detection limit for recombinant GST tagged RUNX1T1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RUNX1T1/ETO Antibody (5A12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RUNX1T1 (NP_004340, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EEIWKKAEEAVNEVKRQAMTELQKAVSEAERKAHDMITTERAKMERTVAEAKRQAAEDALAVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHICG
• Specificity: RUNX1T1 - runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RUNX1T1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RUNX1T1/ETO Antibody (5A12) - Azide and BSA Free

• AML1T1protein CBFA2T1
• CBFA2T1Cyclin-D-related protein
• CDRMGC2796
• core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclinD-related
• DKFZp564B213
• Eight twenty one protein
• ETOFLJ33145
• MTG8acute myelogenous leukemia 1 translocation 1, cyclin-D related
• Protein ETO
• Protein MTG8
• runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
• Zinc finger MYND domain-containing protein 2
• ZMYND2myeloid translocation gene on 8q22

Background

The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RUNX1T1
• Entrez:
  • 862(Human)
• OMIM:
  • 133435(Human)
• Uniprot:
  • NP_004340(Human)