RUFY1 Antibody

Images

 
Western Blot: RUFY1 Antibody [NBP1-68865] - NBP1-68865 (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

RUFY1 Antibody Summary

Immunogen
Peptide with sequence C-QKNEAITSFEGKT corresponding to internal region according to NP_001035541.1.
Epitope
C-QKNEAITSFEGKT
Specificity
This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RUFY1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 - 0.2 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 85 kDa band observed in HeLa lysates (calculated MW of 79.8 kDa band according to NP_079434.3).
Publications
Read Publication using NBP1-68865.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RUFY1 Antibody

  • La-binding protein 1
  • Rab4-interacting protein
  • RABIP4FYVE-finger protein EIP1
  • RUN and FYVE domain containing 1
  • RUN and FYVE domain-containing 1
  • RUN and FYVE domain-containing protein 1
  • ZFYVE12FLJ22251
  • Zinc finger FYVE domain-containing protein 12

Background

RUFY1 contains 1 FYVE-type zinc finger and 1 RUN domain. It binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RUFY1 Antibody (NBP1-68865)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RUFY1
Entrez
Uniprot