RSK2 Antibody (2G10) - Azide and BSA Free

Western Blot: RSK2 Antibody (2G10) [H00006197-M01] - RPS6KA3 monoclonal antibody (M01), clone 2G10. Analysis of RPS6KA3 expression in HepG2.

Immunocytochemistry/Immunofluorescence: RSK2 Antibody (2G10) [H00006197-M01] - Analysis of monoclonal antibody to RPS6KA3 on HeLa cell . Antibody concentration 10 ug/ml.

Proximity Ligation Assay: RSK2 Antibody (2G10) [H00006197-M01] - Analysis of protein-protein interactions between MAPK3 and RPS6KA3. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF
• Clone: 2G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RSK2 Antibody (2G10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RPS6KA3 (NP_004577, 2 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEEVSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGSDARQ
• Specificity: RPS6KA3 - ribosomal protein S6 kinase, 90kDa, polypeptide 3 (2G10)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KA3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RSK2 Antibody (2G10) - Azide and BSA Free

• 90 kDa ribosomal protein S6 kinase 3
• CLS
• Coffin-Lowry syndrome
• EC 2.7.11
• EC 2.7.11.1
• HU-3
• Insulin-stimulated protein kinase 1
• ISPK1
• ISPK-1
• MAP kinase-activated protein kinase 1b
• MAPK-activated protein kinase 1b
• MAPKAP kinase 1b
• MAPKAPK1B
• MAPKAPK-1b
• mental retardation, X-linked 19
• MRX19
• p90-RSK2
• p90RSK3
• pp90RSK2
• ribosomal protein S6 kinase alpha-3
• ribosomal protein S6 kinase, 90kD, polypeptide 3
• ribosomal protein S6 kinase, 90kDa, polypeptide 3
• Ribosomal S6 kinase 2
• RPS6KA3
• RSK
• RSK2
• RSK-2
• RSK2p90-RSK 3
• S6K-alpha3
• S6K-alpha-3

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA3
• Entrez:
  • 6197(Human)
• Uniprot:
  • NP_004577(Human)