RSK1 Antibody

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.25 mg/ml

Order Details

RSK1 Antibody Summary

Immunogen
Synthetic peptide corresponding to the c-terminal of human p90 RSK1 protein conjugated to KLH.
Specificity
p90 RSK1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6KA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100-1:250
Application Notes
This antibody can be used in Western Blot, Immunoprecipitation. *Optimal dilutions should be determined by the end user.
Positive Control
Brain Lysate (NB820-59657)
RSK1 Lysate (NBL1-15570)

Reactivity Notes

Cross-reacts with Human, Mouse, Rat and Bovine. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris
Preservative
0.05% Sodium Azide
Concentration
0.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RSK1 Antibody

  • 90kD, polypeptide 1)
  • EC 2.7.11
  • EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1
  • HU-1
  • MAP kinase-activated protein kinase 1a
  • MAPK-activated protein kinase 1a
  • MAPKAP kinase 1a
  • MAPKAPK-1a
  • MAPKAPL1A
  • p90RSK1
  • p90S6K
  • ribosomal protein S6 kinase alpha 1
  • ribosomal protein S6 kinase alpha-1
  • ribosomal protein S6 kinase, 90kD, polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 1
  • Ribosomal S6 kinase 1
  • RPS6KA1
  • RSK
  • RSK1
  • RSK-1
  • RSK1p90-RSK 1
  • S6K-alpha 1
  • S6K-alpha-1

Background

The p90 Ribosomal S6 kinases (RSKs) comprise a family of unique protein serine/threonine kinases that lie downstream of the mitogen activated protein kinases, predominantely ERK. Unlike other kinases however, the RSK family (RSK1, RSK2, RSK3 and RSK4) possess two kinase domains. Activation of ERK results in the phosphorylation and subsequent activation of RSKs. In turn, RSKs translocate to the nucleus where they phosphorylate a diverse range of nuclear substrates, many of which include transcription factors like c-Fos, estrogen receptor, NFkappaB/IkappaBalpha, cAMP-response element-binding protein (CREB) and CREB-binding protein. Consequently, the RSK family plays critical roles in mediating a number of biological responses including cell cycle regulation and protein synthesis, amongst others. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPS6KA1
Entrez