RSK1 Antibody

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, TS1 mouse helper T cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF ...read more
RSK1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human/Mouse/Rat RSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF992) at 5 µg/mL overnight ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

RSK1 Antibody Summary

Immunogen
E. coli-derived recombinant human RSK1
Accession # Q15418
Specificity
Detects human, rat and mouse RSK1. This antibody does not detect recombinant human RSK2.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6KA1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publication using
AF992 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RSK1 Antibody

  • 90kD, polypeptide 1)
  • EC 2.7.11
  • EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1
  • HU-1
  • MAP kinase-activated protein kinase 1a
  • MAPK-activated protein kinase 1a
  • MAPKAP kinase 1a
  • MAPKAPK-1a
  • MAPKAPL1A
  • p90RSK1
  • p90S6K
  • ribosomal protein S6 kinase alpha 1
  • ribosomal protein S6 kinase alpha-1
  • ribosomal protein S6 kinase, 90kD, polypeptide 1
  • ribosomal protein S6 kinase, 90kDa, polypeptide 1
  • Ribosomal S6 kinase 1
  • RPS6KA1
  • RSK
  • RSK1
  • RSK-1
  • RSK1p90-RSK 1
  • S6K-alpha 1
  • S6K-alpha-1

Background

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC

Publications for RSK1 Antibody (AF992)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Video Protocols

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Bioinformatics Tool for RSK1 Antibody (AF992)

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Bioinformatics

Gene Symbol RPS6KA1
Uniprot