RSK1 Antibody (1019B) [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 1019B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 700

RSK1 Antibody (1019B) [Alexa Fluor® 700] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human RSK1 S221 site
• Specificity: Detects human and mouse RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RSK1 Antibody (1019B) [Alexa Fluor® 700]

• 90kD, polypeptide 1)
• EC 2.7.11
• EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1
• HU-1
• MAP kinase-activated protein kinase 1a
• MAPK-activated protein kinase 1a
• MAPKAP kinase 1a
• MAPKAPK-1a
• MAPKAPL1A
• p90RSK1
• p90S6K
• ribosomal protein S6 kinase alpha 1
• ribosomal protein S6 kinase alpha-1
• ribosomal protein S6 kinase, 90kD, polypeptide 1
• ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Ribosomal S6 kinase 1
• RPS6KA1
• RSK
• RSK1
• RSK-1
• RSK1p90-RSK 1
• S6K-alpha 1
• S6K-alpha-1

Background

RSK1 (Ribosomal S6 Kinase 1), gene name RPS6KA1 (Ribosomal Protein S6 Kinase alpha 1), also known as p90RSK1 (90 kDa RSK1) or MAPKAPK 1a (MAPK Activated Protein Kinase 1a), is a widely expressed member of the RSK family of growth factor-regulated serine/threonine kinases. RSK proteins contain two non-identical kinase catalytic domains, and mediate activation of Mitogen-Activated Kinase (MAPK) cascades and stimulation of cell proliferation and differentiation. Interaction with Erk1 and Erk2 initiates an ordered phosphorylation sequence which regulates RSK activation.  Phosphorylation of Ser 380 recruites PDK1 which, in turn, phosphorylates RSK1 in the activation loop at Ser 221 (or Ser227 in RSK2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.