RSK Antibody (792921) - Pan Specific

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat RSK Pan Specific Monoclonal Antibody (Catalog # MAB2056) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RSK at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for RSK at approximately 86 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat RSK Pan Specific Monoclonal Antibody (Catalog # MAB2056). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 792921
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

RSK Antibody (792921) - Pan Specific Summary

• Immunogen: synthetic peptide corresponding to Lys171-Asp193 of human RSK2
  Accession # P51821
• Specificity: Detects human, mouse, and rat RSK1, 2, 3, and 4 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RSK Antibody (792921) - Pan Specific

• HU-1;MAPKAPK1;MAPKAPK1A;p90Rsk;Ribosomal protein S6 kinase alpha-1;RPS6KA1;RSK;RSK1
• RSK

Background

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. Within the range used as immunogen, human RSK2 shares 96%, 100%, and 91% amino acid sequence identity with RSK1, RSK3, RSK4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RSK Antibody (MAB2056)(3)

Publications using MAB2056

• Abe, R J, Savage, H Et al. p90RSK-MAGI1 Module Controls Endothelial Permeability by Post-translational Modifications of MAGI1 and Hippo Pathway. Front Cardiovasc Med 12/12/2020 [PMID: 33304925]
• Paez-Mayorga J, Chen AL, Kotla S et al. Ponatinib Activates an Inflammatory Response in Endothelial Cells via ERK5 SUMOylation. Front Cardiovasc Med 9/6/2018 [PMID: 30238007]
• Kyung Ae Ko, Yin Wang, Sivareddy Kotla, Yuka Fujii, Hang Thi Vu, Bhanu P. Venkatesulu et al. Developing a Reliable Mouse Model for Cancer Therapy-Induced Cardiovascular Toxicity in Cancer Patients and Survivors Frontiers in Cardiovascular Medicine 2018-04-05 [PMID: 29675417] (Immunohistochemistry, Transgenic Mouse)

Bioinformatics

• Uniprot:
  • P51821()