RRN3 Antibody

Images

 
Western Blot: RRN3 Antibody [NBP1-71822] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-TIF-IA antibody used for WB at ...read more
Western Blot: RRN3 Antibody [NBP1-71822] - 20ug of WCE of 293T or NIH cells from a review by a confirmed customer.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

RRN3 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 601 and 651 of human Transcription Initiation Factor IA using the numbering given in entry NP_060897.3 (GeneID 54700).
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RRN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 mcg/mg
Reviewed Applications
Read 1 Review rated 4
using
NBP1-71822 in the following applications:

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee and Northern white-cheeked gibbon.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RRN3 Antibody

  • DKFZp566E104
  • RNA polymerase I-specific transcription initiation factor RRN3
  • RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
  • RRN3 RNA polymerase I transcription factor homolog (yeast)
  • TIF-IA
  • TIFIAMGC104238
  • Transcription initiation factor IA
  • transcription initiation factor TIF-IA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for RRN3 Antibody (NBP1-71822) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-71822:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot RRN3 NBP1-71822
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reviewed by:
Anony- mous
WB Human 11/15/2013
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Summary

ApplicationWestern Blot
Sample Tested293T and NIH cells, transfected
SpeciesHuman
LotLot 1
CommentsIt is very specific to hRRN3 however there are some non-specific bands that appear when diluted in BSA. In milk, the blot is clean however I was unable to see the endogenous RRN3 in 293T cells even after 15mins exposure, just the transfected track lit up. 20ug of whole cell extracts (SDS lysis buffer) from either 293T or NIH cells, transfected with mouse or human RRN3, were loaded on an 8% SDS-PAGE gel. IB using NBP1-71822 1:5000 dilution in either 5% BSA or 5% milk in TBS-T (0.01% Tween) incubated overnight at 4C. NBP1-71822 is very specific to human RRN3 and does not recognize endogenous or overexpressed mouse RRN3/TIF-IA.

Blocking

Blocking Details5% BSA or 5% milk in TBS-T (0.01% tween)

Primary Anitbody

Dilution Ratio1:5,000 overnight at 4C

Comments

CommentsIt is very specific to hRRN3 however there are some non-specific bands that appear when diluted in BSA. In milk, the blot is clean however I was unable to see the endogenous RRN3 in 293T cells even after 15mins exposure, just the transfected track lit up. 20ug of whole cell extracts (SDS lysis buffer) from either 293T or NIH cells, transfected with mouse or human RRN3, were loaded on an 8% SDS-PAGE gel. IB using NBP1-71822 1:5000 dilution in either 5% BSA or 5% milk in TBS-T (0.01% Tween) incubated overnight at 4C. NBP1-71822 is very specific to human RRN3 and does not recognize endogenous or overexpressed mouse RRN3/TIF-IA.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RRN3 Antibody (NBP1-71822) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for RRN3 Antibody (NBP1-71822)

Discover related pathways, diseases and genes to RRN3 Antibody (NBP1-71822). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Anony- mous
11/15/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol RRN3
Uniprot