RPS3A Antibody - BSA Free

Western Blot: RPS3A Antibody [NBP1-31598] - PS3A antibody detects RPS3A protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract. 12 % SDS-PAGE. RPS3A antibody dilution: 1:1000

Immunocytochemistry/Immunofluorescence: RPS3A Antibody [NBP1-31598] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: RPS3A Antibody [NBP1-31598] - Mouse brain stem. RPS3A antibody dilution: 1:200. Antigen Retrieval: Trilogy (EDTA based, pH 8.0) buffer, 15min.

Western Blot: RPS3A Antibody [NBP1-31598] - Sample(30 ug whole cell lysate)A: 293T B: A431 C:H1299 D:HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.

Western Blot: RPS3A Antibody [NBP1-31598] - RPS3A antibody detects RPS3A protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGERPS3A antibody dilution: 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPS3A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RPS3A. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS3A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
• Application Notes: Immunoprecipitation Assay dependent
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RPS3A Antibody - BSA Free

• 40S ribosomal protein S3a
• FTE1
• fte-1
• MFTL
• MGC23240
• ribosomal protein S3A
• v-fos transformation effector protein 1
• v-fos transformation effector protein

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RPS3A