RPS21 Antibody - BSA Free

Western Blot: RPS21 Antibody [NBP1-87100] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunocytochemistry/Immunofluorescence: RPS21 Antibody [NBP1-87100] - Staining of human cell line U-251 MG shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: RPS21 Antibody [NBP1-87100] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Western Blot: RPS21 Antibody [NBP1-87100] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunohistochemistry-Paraffin: RPS21 Antibody [NBP1-87100] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.

Immunohistochemistry-Paraffin: RPS21 Antibody [NBP1-87100] - Staining of human skin shows strong cytoplasmic positivity in a subset of squamous epithelial cells.

Immunohistochemistry-Paraffin: RPS21 Antibody [NBP1-87100] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPS21 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RPS21 Antibody - BSA Free (NBP1-87100) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RPS21 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RPS21 Recombinant Protein Antigen (NBP1-87100PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RPS21 Antibody - BSA Free

• ribosomal protein S21,40S ribosomal protein S21,8.2 kDa differentiation factor

Background

RPS21 is a gene that codes for a ribosomal protein that is a component of the 40S subunit, and has a length of 83 amino acids and a weight of approximately 9 kDa. Current studies are being done on several disorders and diseases related to this gene, including pure red-cell aplasia, anemia, malaria, and leukemia. RPS21 has also been shown to have interactions with RIOK2, RPSA, ABCF2, KRR1, and MCTS1 in pathways such as the cytoplasmic ribosomal protein, PB1-F2 synthesis, influenza life cycle, and viral mRNA translation pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RPS21 Antibody (NBP1-87100)(1)

Publication using NBP1-87100

• Badhai J, Frojmark AS, Razzaghian HR et al. Posttranscriptional down regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency. FEBS Lett 2009-06-18 [PMID: 19454283]

Bioinformatics

• Gene Symbol: RPS21