RPB9 Antibody (2B3) - Azide and BSA Free

Sandwich ELISA: RPB9 Antibody (2B3) [H00005438-M01] - Detection limit for recombinant GST tagged POLR2I is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RPB9 Antibody (2B3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: POLR2I (AAH17112, 26 a.a. ~ 125 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE
• Specificity: POLR2I - polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR2I
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RPB9 Antibody (2B3) - Azide and BSA Free

• DNA-directed RNA polymerase II 14.5 kDa polypeptide
• DNA-directed RNA polymerase II subunit I
• DNA-directed RNA polymerase II subunit RPB9
• hRPB14.5
• polymerase (RNA) II (DNA directed) polypeptide I (14.5kD)
• polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• RNA polymerase II 14.5 kDa subunit
• RNA polymerase II subunit B9
• RPB14.5
• RPB9

Background

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2I
• Entrez:
  • 5438(Human)
• OMIM:
  • 180662(Human)
• Uniprot:
  • AAH17112(Human)